PMID- 12187281
OWN - NLM
STAT- MEDLINE
DCOM- 20020918
LR  - 20230302
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 168
IP  - 3
DP  - 2002 Sep
TI  - Activation of mitogen activated protein kinases and apoptosis of germ cells after 
      vasectomy in the rat.
PG  - 1273-8
AB  - PURPOSE: Vasectomy induces a large amount of germ cell apoptosis. We examined the 
      activation of mitogen activated protein kinases (MAPKs) in association with the 
      apoptosis and proliferation of germ cells after vasectomy in the rat. MATERIALS 
      AND METHODS: Eight-week-old Wistar rats underwent bilateral vasectomy and the 
      testes were harvested 1 to 9 days after vasectomy. Germ cell apoptosis was 
      evaluated by terminal deoxynucleotidyl transferase mediated deoxyuridine 
      triphosphate nick end labeling and electrophoretic assay of DNA fragmentation. 
      Western blotting and immunohistochemistry were used to examine the temporal and 
      spatial activation of signal regulated kinases 1/2, c-Jun-terminal kinases 1/2 
      and p38. Phospho-specific MAPK antibodies were used to examine their activations. 
      Proliferation of germ cells was evaluated by proliferative nuclear cell antigen 
      expression. RESULTS: Germ cell apoptosis was detected predominantly in primary 
      spermatocytes with a peak 7 days after vasectomy. Signal regulated kinases 1/2, 
      c-Jun-terminal kinases 1/2 and p38 were constitutively expressed in the control 
      testis. Western blotting and immunohistochemistry showed rapid activation of 
      signal regulated kinases 1/2, followed by activation of c-Jun-terminal kinases 
      1/2 and p38. Immunohistochemical study demonstrated the temporal and spatial 
      relationships of apoptosis and MAPK activation in primary spermatocytes. On the 
      other hand, proliferating cell nuclear antigen expression was enhanced in 
      tetraploid spermatocyte and spermatogonia maximally 5 days after vasectomy. 
      CONCLUSIONS: MAPKs were rapidly activated after vasectomy and germ cell apoptosis 
      was observed after vasectomy. In contrast to the delayed phase up to 24 weeks 
      after vasectomy, we observed hyperdynamic cellular turnover, spermatocyte loss 
      through apoptosis and enhanced germ cell proliferation transiently at the early 
      phase after vasectomy.
FAU - Shiraishi, Koji
AU  - Shiraishi K
AD  - Departments of Urology and Legal Medicine, Yamaguchi University School of 
      Medicine, Yamaguchi, Japan.
FAU - Yoshida, Ken-Ichi
AU  - Yoshida K
FAU - Fujimiya, Tatsuya
AU  - Fujimiya T
FAU - Naito, Katsusuke
AU  - Naito K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Germ Cells/chemistry/*cytology
MH  - In Situ Nick-End Labeling
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Male
MH  - Mitogen-Activated Protein Kinases/analysis/*metabolism
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Seminiferous Epithelium/chemistry/cytology
MH  - Seminiferous Tubules/cytology
MH  - Spermatocytes/chemistry/cytology
MH  - *Vasectomy
EDAT- 2002/08/21 10:00
MHDA- 2002/09/19 10:01
CRDT- 2002/08/21 10:00
PHST- 2002/08/21 10:00 [pubmed]
PHST- 2002/09/19 10:01 [medline]
PHST- 2002/08/21 10:00 [entrez]
AID - S0022-5347(05)64639-3 [pii]
AID - 10.1016/S0022-5347(05)64639-3 [doi]
PST - ppublish
SO  - J Urol. 2002 Sep;168(3):1273-8. doi: 10.1016/S0022-5347(05)64639-3.