PMID- 12188502
OWN - NLM
STAT- MEDLINE
DCOM- 20020909
LR  - 20190916
IS  - 0301-620X (Print)
IS  - 0301-620X (Linking)
VI  - 84
IP  - 5
DP  - 2002 Jul
TI  - Bone-resorptive effects of endotoxin-contaminated high-density polyethylene 
      particles spontaneously eliminated in vivo.
PG  - 767-73
AB  - Wear particles commonly used for experiments may carry adherent endotoxin on 
      their surfaces, which may be responsible for the observed effects. In this study, 
      we attached titanium plates to the tibiae of 20 rats. After osseointegration, 
      endotoxin-contaminated or uncontaminated high-density-polyethylene (HDPE) 
      particles were applied. Contaminated specimens showed a dramatic resorption of 
      bone after seven days but new bone filled the site again at 21 days. 
      Uncontaminated specimens showed no resorption. In 18 rats we implanted 
      intramuscularly discs of ultra-high-molecular-weight polyethylene (UHMWPE) with 
      baseline or excess contamination of endotoxin. Excess endotoxin disappeared 
      within 24 hours and the amount of endotoxin remained at baseline level 
      (contamination from production). Uncontaminated titanium discs did not adsorb 
      endotoxin in vivo. The endotoxin was measured by analytical chemistry. 
      Locally-applied endotoxin stimulated bone resorption similarly to that in 
      experiments with wear particles. Endotoxin on the surface of implants and 
      particles appeared to be inactivated in situ. A clean implant surface did not 
      adsorb endotoxin. Our results suggest that endotoxin adhering to orthopaedic 
      implants is not a major cause for concern.
FAU - Skoglund, B
AU  - Skoglund B
AD  - Lund University Hospital and Lund University, Sweden.
FAU - Larsson, L
AU  - Larsson L
FAU - Aspenberg, P A
AU  - Aspenberg PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Joint Surg Br
JT  - The Journal of bone and joint surgery. British volume
JID - 0375355
RN  - 0 (Endotoxins)
RN  - 0 (Polyethylenes)
RN  - 0 (ultra-high molecular weight polyethylene)
SB  - IM
MH  - Animals
MH  - *Bone Resorption
MH  - *Endotoxins
MH  - Equipment Contamination
MH  - Female
MH  - Models, Animal
MH  - Osteolysis/*microbiology
MH  - Polyethylenes/*therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/08/22 10:00
MHDA- 2002/09/11 10:01
CRDT- 2002/08/22 10:00
PHST- 2002/08/22 10:00 [pubmed]
PHST- 2002/09/11 10:01 [medline]
PHST- 2002/08/22 10:00 [entrez]
AID - 10.1302/0301-620x.84b5.11775 [doi]
PST - ppublish
SO  - J Bone Joint Surg Br. 2002 Jul;84(5):767-73. doi: 10.1302/0301-620x.84b5.11775.