PMID- 12188557
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20031114
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 45
IP  - 10
DP  - 2002
TI  - Evaluation of thermophilic anaerobic microbial consortia using fluorescence in 
      situ hybridization (FISH).
PG  - 27-33
AB  - In this study we investigated the development of anaerobic biofilms in 
      differential reactors and suspension cultures in batch reactors under 
      thermophilic (55 degrees C) conditions. FISH, SEM, chemical and chromatographic 
      analysis were used. The differential reactors reached 99.6%, 92.3% and 6.7% of 
      acetic acid, COD and sulfate removal efficiencies, respectively, after 166 h of 
      incubation time. The batch reactor reached 95.6% and 31.8% of acetic acid and 
      sulfate removal efficiencies after 675 h, respectively. FISH results showed that 
      bacterial cells rather than archaeal cells dominated biofilms. These cells, 
      detected with the Bacteria specific probe (EUB338), accounted for 61.1% (+/-3.6) 
      of the DAPI-stained cells and resembled acetate-oxidizing rods and 
      Desulfotomaculum morphologies. Archaeal cells, which hybridized to the Archaea 
      specific probe (ARC915), were also detected in biofilm but they accounted for 
      36.7% (+/-2.9) of the DAPI-stained cells. These cells were similar to 
      Methanosaeta-like and hydrogenotrophic methanogen rods. In the suspension 
      culture, archaeal cells (58.0%+/-3.8) morphologically similar to Methanosarcina 
      and hydrogenotrophic methanogen rods were predominant over bacterial cells 
      (41.0%+/-4.5), which resembled acetate-oxidizing rods and Desulfotomaculum 
      morphologies. The percentage of sulfate-reducing bacteria cells (SRB) ranged from 
      12.2% (+/-2.5) to 21.7% (+/-2.8) in the biofilms and from 13.3% (+/-3.6) to 21.7% 
      (+/-4.3) of the DAPI stained cells in suspension culture.
FAU - Domingues, M R
AU  - Domingues MR
AD  - Departamento de Hidraulica e Saneamento, Escola de Engenharia de Sao Carlos, 
      Universidade de Sao Paulo (USP), Sao Carlos - SP, Brazil. 
      catharanthus@hotmail.com
FAU - Araujo, J C
AU  - Araujo JC
FAU - Varesche, M B A
AU  - Varesche MB
FAU - Vazoller, R F
AU  - Vazoller RF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
SB  - IM
MH  - *Archaea
MH  - *Bacteria, Anaerobic
MH  - Biofilms
MH  - *Bioreactors
MH  - *Euryarchaeota
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Electron, Scanning
MH  - Population Dynamics
MH  - Temperature
EDAT- 2002/08/22 10:00
MHDA- 2003/01/15 04:00
CRDT- 2002/08/22 10:00
PHST- 2002/08/22 10:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/08/22 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2002;45(10):27-33.