PMID- 12189501
OWN - NLM
STAT- MEDLINE
DCOM- 20021121
LR  - 20141120
IS  - 0945-6317 (Print)
IS  - 0945-6317 (Linking)
VI  - 441
IP  - 2
DP  - 2002 Aug
TI  - Complex formation of IQGAP1 with E-cadherin/catenin during cohort migration of 
      carcinoma cells. Its possible association with localized release from cell-cell 
      adhesion.
PG  - 124-32
AB  - In histopathological sections, it is frequently observed that carcinoma cells 
      invade the stroma as coherent cell nests rather than single cells. We have called 
      this type of movement "cohort migration (CM)" and developed an in vitro model, in 
      which human colon carcinoma cells move as coherent cell sheets when stimulated 
      with hepatocyte growth factor/scatter factor (HGF/SF). In this CM model, 
      localized release from cell-cell adhesion at the lower portion of cells is 
      essential for cell movement. Its mechanism was investigated in this study with 
      special reference to the E-cadherin/catenin complex (Ecc) and IQGAP1. IQGAP1 is a 
      target molecule of Cdc42 and Rac1 and negatively regulates the Ecc-based 
      cell-cell adhesion by dissociating alpha-catenin, a key molecule that links Ecc 
      to actin cytoskeleton, from Ecc. In our study, the amount of IQGAP1 bound to Ecc 
      increased in migrating cells in association with a decrease in the alpha-catenin 
      level in Ecc. In accordance with this, IQGAP1 showed a shift from the cytosol to 
      the membrane fraction. Moreover, confocal laser microscopic study demonstrated 
      the localization of IQGAP1 at the membranes of the lower portion of migrating 
      cells, where cell-cell adhesion was specifically disrupted during CM. 
      Furthermore, when HGF/SF-induced CM was enhanced with pre-coated extracellular 
      matrix (ECM) components, the level of IQGAP1 in Ecc increased more than that 
      caused by HGF/SF alone. On the contrary, when CM was inhibited by interrupting 
      cell-ECM interaction, the level of IQGAP1 in Ecc did not increase despite HGF/SF 
      stimulation. Taken together, these results indicate close association of IQGAP1 
      with localized disruption of cell-cell adhesion during CM and that modulation of 
      CM by cross-talk between signals induced by HGF/SF and cell-ECM interactions also 
      involves IQGAP1-related mechanisms.
FAU - Shimao, Yoshiya
AU  - Shimao Y
AD  - Department of Pathology, Miyazaki Medical Collage Hospital, 5200 Kihara, 
      Kiyotake, Miyazaki 889-1692, Japan.
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
FAU - Inoue, Teruhiko
AU  - Inoue T
FAU - Koono, Masashi
AU  - Koono M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020219
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (CTNNA1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Ctnna1 protein, mouse)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (IQ motif containing GTPase activating protein 1)
RN  - 0 (Recombinant Proteins)
RN  - 0 (alpha Catenin)
RN  - 0 (ras GTPase-Activating Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adenocarcinoma/*metabolism/pathology
MH  - Animals
MH  - Cadherins/*metabolism
MH  - Carrier Proteins/*metabolism
MH  - Cell Adhesion/drug effects/physiology
MH  - Cell Movement/drug effects/*physiology
MH  - Colonic Neoplasms
MH  - Cytoskeletal Proteins/*metabolism
MH  - Extracellular Matrix/drug effects/metabolism
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Invasiveness
MH  - Receptor Cross-Talk/drug effects
MH  - Recombinant Proteins
MH  - Tumor Cells, Cultured
MH  - alpha Catenin
MH  - *ras GTPase-Activating Proteins
EDAT- 2002/08/22 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/08/22 10:00
PHST- 2001/08/17 00:00 [received]
PHST- 2001/12/10 00:00 [accepted]
PHST- 2002/08/22 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/08/22 10:00 [entrez]
AID - 10.1007/s00428-002-0603-3 [doi]
PST - ppublish
SO  - Virchows Arch. 2002 Aug;441(2):124-32. doi: 10.1007/s00428-002-0603-3. Epub 2002 
      Feb 19.