PMID- 12191497
OWN - NLM
STAT- MEDLINE
DCOM- 20030211
LR  - 20190826
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 102
IP  - 1-2
DP  - 2002 Jun 15
TI  - In vivo and in vitro localization of brain-derived neurotrophic factor, 
      fibroblast growth factor-2 and their receptors in the bullfrog vestibular end 
      organs.
PG  - 83-99
AB  - The inner ear sensory epithelia of vertebrates are composed mainly of supporting 
      cells and hair cells (HCs). Brain-derived neurotrophic factor (BDNF) and 
      fibroblast growth factor-2 (FGF-2) are trophins that are believed to play an 
      essential role in the development and innervation of inner ear epithelia. Both 
      trophins also may play a crucial role in the maintenance and regeneration of hair 
      cells in the adult vertebrate ear. In the bullfrog vestibular system, hair cells 
      are produced throughout life, and the epithelia regenerates following 
      ototoxicity. The expression of BDNF and FGF-2 in the vestibular organs of the 
      adult bullfrog was investigated at a cellular level both in histological sections 
      and in vitro in dissociated cell cultures. In histological sections of the crista 
      ampullaris, in situ hybridization and immunocytochemical techniques demonstrated 
      that HCs express both BDNF and its receptor trkB, while the supporting cells 
      express the receptor trkB alone. Following dissociation and in vitro cell culture 
      no changes in the pattern of BDNF and trkB receptor were observed. 
      Immunocytochemical studies demonstrated that in vivo hair cells express FGF-2 and 
      the receptors FGFR-1 and FGFR-2 while supporting cells do not express either 
      molecule. Following dissociation, HCs continue to express FGF-2 and its two 
      receptors, while supporting cells upregulate the expression of FGF-2 and its 
      receptor FGFR-2. These data confirm the potential role of BDNF and FGF-2 trophic 
      regulation of the sensory epithelia of the adult inner ear. The findings suggest 
      that BDNF has a role in the maintenance of the vestibular epithelia while FGF-2 
      may regulate the proliferation of supporting cells.
FAU - Cristobal, Ricardo
AU  - Cristobal R
AD  - Victor Goodhill Ear Center, Division of Head and Neck Surgery, University of 
      California, Los Angeles School of Medicine, CHS, Room 62-129, 10833 Le Conte 
      Ave., Los Angeles, CA 90095-1624, USA.
FAU - Popper, Paul
AU  - Popper P
FAU - Lopez, Ivan
AU  - Lopez I
FAU - Micevych, Paul
AU  - Micevych P
FAU - De Vellis, Jean
AU  - De Vellis J
FAU - Honrubia, Vicente
AU  - Honrubia V
LA  - eng
GR  - DC00008/DC/NIDCD NIH HHS/United States
GR  - DC01404/DC/NIDCD NIH HHS/United States
GR  - DC02952/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Autocrine Communication/physiology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Communication/physiology
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Hair Cells, Vestibular/cytology/metabolism
MH  - Immunohistochemistry
MH  - Labyrinth Supporting Cells/cytology/metabolism
MH  - Rana catesbeiana/anatomy & histology/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 1
MH  - Receptor, Fibroblast Growth Factor, Type 2
MH  - Receptor, trkB/*metabolism
MH  - Receptors, Fibroblast Growth Factor/*metabolism
MH  - Vestibule, Labyrinth/cytology/*metabolism
EDAT- 2002/08/23 10:00
MHDA- 2003/02/13 04:00
CRDT- 2002/08/23 10:00
PHST- 2002/08/23 10:00 [pubmed]
PHST- 2003/02/13 04:00 [medline]
PHST- 2002/08/23 10:00 [entrez]
AID - S0169328X02002024 [pii]
AID - 10.1016/s0169-328x(02)00202-4 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2002 Jun 15;102(1-2):83-99. doi: 
      10.1016/s0169-328x(02)00202-4.