PMID- 12193542
OWN - NLM
STAT- MEDLINE
DCOM- 20020923
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 143
IP  - 9
DP  - 2002 Sep
TI  - Hepatocyte growth factor/scatter factor facilitates migration of GN-11 
      immortalized LHRH neurons.
PG  - 3306-15
AB  - The molecular cues regulating the migratory process of LHRH neurons from the 
      olfactory placode into the brain are not well known, but gradients of chemotropic 
      and chemorepellent factors secreted by the targets are likely to play a key role 
      in guidance mechanisms. Hepatocyte growth factor/scatter factor (HGF/SF) is a 
      pleiotropic cytokine inducing cell migration. It is involved in a variety of 
      developmental processes through interaction with its receptor c-Met. Here we show 
      that c-Met-antibody labels LHRH migrating neurons in the olfactory mesenchyme of 
      E12 mouse and analyze the potential chemotropic effect of HGF/SF on two 
      immortalized LHRH cell lines, GT1-7 and GN11, isolated from tumors developed in 
      the hypothalamus and in the olfactory bulb, respectively. By RT-PCR analysis, 
      Western blotting, and immunocytochemistry, we provide evidence for a high level 
      of c-Met expression in GN11, but not in GT1-7, cells. In addition, HGF/SF 
      treatment promotes specific migratory activity of GN11 cells, as demonstrated by 
      collagen gel assay, time-lapse video microscopy, and Boyden's chamber 
      experiments. Such promotion is inhibited by the neutralizing antibody. The data 
      reported here represent the first direct evidence of a chemotactic effect of 
      HGF/SF on immortalized LHRH neurons.
FAU - Giacobini, P
AU  - Giacobini P
AD  - Department of Human and Animal Biology, University of Turin, 10123 Torino, Italy.
FAU - Giampietro, C
AU  - Giampietro C
FAU - Fioretto, M
AU  - Fioretto M
FAU - Maggi, R
AU  - Maggi R
FAU - Cariboni, A
AU  - Cariboni A
FAU - Perroteau, I
AU  - Perroteau I
FAU - Fasolo, A
AU  - Fasolo A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/cytology
MH  - Brain Neoplasms
MH  - Cell Line, Transformed
MH  - Cell Movement/*physiology
MH  - Chemotaxis
MH  - Collagen
MH  - Gonadotropin-Releasing Hormone/analysis/*metabolism
MH  - Hepatocyte Growth Factor/pharmacology/*physiology
MH  - Hypothalamic Neoplasms
MH  - Immunohistochemistry
MH  - Immunosorbent Techniques
MH  - Mice
MH  - Microscopy, Video
MH  - Neurons/*physiology
MH  - Olfactory Bulb
MH  - Olfactory Mucosa/chemistry/cytology/embryology
MH  - Proto-Oncogene Proteins c-met/analysis/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 2002/08/24 10:00
MHDA- 2002/09/24 06:00
CRDT- 2002/08/24 10:00
PHST- 2002/08/24 10:00 [pubmed]
PHST- 2002/09/24 06:00 [medline]
PHST- 2002/08/24 10:00 [entrez]
AID - 10.1210/en.2002-220146 [doi]
PST - ppublish
SO  - Endocrinology. 2002 Sep;143(9):3306-15. doi: 10.1210/en.2002-220146.