PMID- 1219368
OWN - NLM
STAT- MEDLINE
DCOM- 19760510
LR  - 20061115
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 9
IP  - 1
DP  - 1975 Jan-Feb
TI  - [Early changes in liver chromatin in response to partial hepatectomy].
PG  - 138-44
AB  - Guinea-pig and mouse liver chromatin responds to the partial hepatectomy by an 
      increase in binding of a basic dye acridine orange (AO) and by a decrease of its 
      stability to heat in thermal denaturation test in situ. Degree of the changes in 
      AO chromatin binding is identical in the cells of different ploidy and 
      proportional to their DNA content. Treatment of the preparations by 0.6 M NaCl 
      solutions under conditions bringing about the selective removal of histone H1 
      from the cells produces in vitro changes in DNA properties taking place in cells 
      in vivo in the course of their activation. The treatment of cells with 0.35 M 
      NaCl solution results in the disappearance of changes occurring in the chromatin 
      of activated cells whereas the properties of control cells remain unchanged. The 
      data obtained are interpreted as a result of the removal of some non-histone 
      regulatory proteins from the chromatin of activated cells that is accompanied by 
      changes in the character of DNA-histone interaction. At the time of maximum 
      increase of AO binding a significant intensification of endogenous RNA polymerase 
      activity was found, the incorporation of [3H] UTP in the nucleolus being higher 
      than that in the extranucleolar part of the nucleus. High ionic strength in the 
      incubation medium (0.4 M (NH4)2SO4) results in drastic increase of radioactive 
      label in the nucleus and in the disappearance of differences between activated 
      and non-activated chromatin. It is concluded that the intensification of RNA 
      synthesis under the influence of proliferative stimulus is more likely dependent 
      on the additional opening of DNA-matrix than on the direct activation of the 
      enzyme.
FAU - Kushch, A A
AU  - Kushch AA
FAU - Kolesnikov, V A
AU  - Kolesnikov VA
FAU - Zelenin, A V
AU  - Zelenin AV
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Izmeneniia svoistv khromatina kletok pecheni v rannie sroki posle chastichnoi 
      gepatektomii.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.7.6 (DNA-Directed RNA Polymerases)
SB  - IM
MH  - Animals
MH  - Chromatin/*metabolism/ultrastructure
MH  - DNA/metabolism
MH  - DNA-Directed RNA Polymerases/metabolism
MH  - Guinea Pigs
MH  - Hepatectomy
MH  - Histones/metabolism
MH  - Liver/*metabolism
MH  - *Liver Regeneration
MH  - Microscopy, Electron
MH  - Osmolar Concentration
MH  - Protein Binding
MH  - Species Specificity
EDAT- 1975/01/01 00:00
MHDA- 1975/01/01 00:01
CRDT- 1975/01/01 00:00
PHST- 1975/01/01 00:00 [pubmed]
PHST- 1975/01/01 00:01 [medline]
PHST- 1975/01/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1975 Jan-Feb;9(1):138-44.