PMID- 1219390
OWN - NLM
STAT- MEDLINE
DCOM- 19760423
LR  - 20130326
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 9
IP  - 3
DP  - 1975 May-Jun
TI  - [The comparative analysis of interaction between acridine orange and 
      DNA-containing systems].
PG  - 378-87
AB  - The interaction between acridine orange (AO) and diluted and concentrated 
      solutions of DNA, DNP systems and chromatin suspension at the physiologic ionic 
      strength was investigated. The effect of AO on DNP systems was also investigated. 
      It was shown that highest possible number of AO molecules bound to DNA made up 
      70% of the total number of nucleotides. The model of AO binding to DNA is 
      proposed and used for calculation of constants of stronger and weaker AO-binding 
      capacities equal to 6-10(6) M-1 and 1,7-10(5) M-1, respectively. The AO-DNA 
      binding constants in DNP-complex are five as low. The primary number of binding 
      sites in chromatin suspension made up 10% of the corresponding sites in DNA and 
      increased as AO was adsorbed. AO induced the supercontraction of oriented DNP 
      systems at the physiologic ionic strength and the appearance of the 
      low-temperature melting hump.
FAU - Karpukhin, A V
AU  - Karpukhin AV
FAU - Spitkovskii, D M
AU  - Spitkovskii DM
FAU - Fedorova, K N
AU  - Fedorova KN
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Sravnitel'nyi analiz vzaimodeistviia akridinovogo oranzhevogo s 
      DNK-soderzhashchimi sistemami.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Acridines)
RN  - 0 (Chromatin)
RN  - 0 (Deoxyribonucleoproteins)
RN  - 0 (Indicators and Reagents)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Acridines
MH  - Binding Sites
MH  - Chromatin
MH  - *DNA
MH  - Deoxyribonucleoproteins
MH  - *Indicators and Reagents
MH  - Kinetics
MH  - Mathematics
MH  - Osmolar Concentration
MH  - Protein Binding
EDAT- 1975/05/01 00:00
MHDA- 1975/05/01 00:01
CRDT- 1975/05/01 00:00
PHST- 1975/05/01 00:00 [pubmed]
PHST- 1975/05/01 00:01 [medline]
PHST- 1975/05/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1975 May-Jun;9(3):378-87.