PMID- 12194042
OWN - NLM
STAT- MEDLINE
DCOM- 20021001
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 373
IP  - 8
DP  - 2002 Apr
TI  - A piezoelectric immunoassay based on self-assembled monolayers of cystamine and 
      polystyrene sulfonate for determination of Schistosoma japonicum antibodies.
PG  - 803-9
AB  - A piezoelectric immunosensor based on an improved immobilization strategy 
      combining self-assembled monolayers (SAM) of cystamine (Cys) and polystyrene 
      sulfonate (PSS) has been developed for the determination of Schistosoma japonicum 
      antibodies (SjAb) in rabbit serum. Cys SAM were first applied to the gold 
      electrode surface of the crystal, serving as a positively-charged base. 
      Schistosoma japonicum antigen (SjAg) was then electrostatically immobilized on 
      the crystal by means of a negatively-charged PSS layer. When sealed by use of an 
      appropriately selected blocking reagent for BSA and normal rabbit serum (NRS), 
      non-specific adsorption could be substantially reduced. The immunosensor was used 
      to determine SjAb in optimized buffer medium with addition of poly(ethylene 
      glycol) (PEG), which served as an immunoreaction enhancer. It was shown 
      experimentally that SjAg immobilized by the Cys-PSS adsorption procedure had 
      higher immunological activity or binding efficiency than those immobilized by the 
      glutaraldehyde (GLU) binding or direct attachment procedures. The immunosensor 
      developed had satisfactory sensitivity and detection limit, and regeneration of 
      the piezoelectric quartz-crystal was easy. Analytical results obtained with 
      infected rabbit serum samples indicated that the proposed immunosensor is a 
      promising alternative for qualitative and quantitative determination of SjAb in 
      clinical diagnosis of infection with Schistosoma japonicum.
FAU - Wang, Cunchang
AU  - Wang C
AD  - Chenzhou Teachers' College, Chenzhou, Hunan, 423000, People's Republic of China.
FAU - Wang, Hua
AU  - Wang H
FAU - Wu, Zhaoyang
AU  - Wu Z
FAU - Shen, Guoli
AU  - Shen G
FAU - Yu, Ruqin
AU  - Yu R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020723
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Polystyrenes)
RN  - 70KO0R01RY (polystyrene sulfonic acid)
RN  - R110LV8L02 (Cystamine)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Antibodies, Helminth/*analysis
MH  - Cystamine/*chemistry
MH  - Immunoassay/*methods
MH  - Polystyrenes/*chemistry
MH  - Schistosoma japonicum/*immunology
EDAT- 2002/08/24 10:00
MHDA- 2002/10/03 04:00
CRDT- 2002/08/24 10:00
PHST- 2002/02/04 00:00 [received]
PHST- 2002/05/14 00:00 [revised]
PHST- 2002/05/29 00:00 [accepted]
PHST- 2002/08/24 10:00 [pubmed]
PHST- 2002/10/03 04:00 [medline]
PHST- 2002/08/24 10:00 [entrez]
AID - 10.1007/s00216-002-1389-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2002 Apr;373(8):803-9. doi: 10.1007/s00216-002-1389-5. Epub 
      2002 Jul 23.