PMID- 12196394
OWN - NLM
STAT- MEDLINE
DCOM- 20030227
LR  - 20210915
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 161
IP  - 4
DP  - 2002 Aug
TI  - Meiotic silencing by unpaired DNA: properties, regulation and suppression.
PG  - 1483-95
AB  - In Neurospora, a gene not paired with a homolog in prophase I of meiosis 
      generates a signal that transiently silences all sequences homologous to it by a 
      process called meiotic silencing by unpaired DNA (MSUD). Thus a deletion mutation 
      in a heterozygous cross is formally "ascus-dominant" because its unpaired 
      wild-type partner silences itself. We describe in detail the isolation of a 
      mutation, Sad-1(UV), that suppresses the dominance of various ascus-dominant 
      mutations. Additional dominant, semidominant, and recessive Sad-1 alleles have 
      been generated by RIP; the DNA of the dominant RIP alleles becomes methylated, 
      but dim-2-dependent methylation is not necessary for silencing. The barrenness of 
      homozygous Sad-1 crosses is not due to the failure to silence unpaired 
      mating-type mat A-2 mat A-3 genes. Transcripts of sad-1(+) can be detected during 
      the sexual phase in a homozygous wild-type cross, indicating that the gene is 
      expressed even if all DNA can pair normally. Meiotic silencing is confined to the 
      ascus in which DNA is unpaired, and silencing does not spread to neighboring asci 
      in a fruiting body of mixed genetic constitution.
FAU - Shiu, Patrick K T
AU  - Shiu PK
AD  - Department of Biological Sciences, Stanford University, Stanford, California 
      94305-5020, USA.
FAU - Metzenberg, Robert L
AU  - Metzenberg RL
LA  - eng
GR  - GM08995/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SAD1 protein, S pombe)
RN  - 0 (Schizosaccharomyces pombe Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - *Gene Expression Regulation, Fungal
MH  - *Gene Silencing
MH  - Homeodomain Proteins/genetics
MH  - *Meiosis
MH  - Molecular Sequence Data
MH  - Neurospora/genetics
MH  - Repressor Proteins/genetics
MH  - Schizosaccharomyces pombe Proteins/genetics
PMC - PMC1462203
EDAT- 2002/08/28 10:00
MHDA- 2003/02/28 04:00
PMCR- 2002/11/01
CRDT- 2002/08/28 10:00
PHST- 2002/08/28 10:00 [pubmed]
PHST- 2003/02/28 04:00 [medline]
PHST- 2002/08/28 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 10.1093/genetics/161.4.1483 [doi]
PST - ppublish
SO  - Genetics. 2002 Aug;161(4):1483-95. doi: 10.1093/genetics/161.4.1483.