PMID- 12196567
OWN - NLM
STAT- MEDLINE
DCOM- 20020913
LR  - 20200225
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 17
DP  - 2002 Sep 1
TI  - Brain-derived neurotrophic factor triggers transcription-dependent, late phase 
      long-term potentiation in vivo.
PG  - 7453-61
AB  - Acute intrahippocampal infusion of brain-derived neurotrophic factor (BDNF) leads 
      to long-term potentiation (BDNF-LTP) of synaptic transmission at medial perforant 
      path-->granule cell synapses in the rat dentate gyrus. Endogenous BDNF is 
      implicated in the maintenance of high-frequency stimulation-induced LTP 
      (HFS-LTP). However, the relationship between exogenous BDNF-LTP and HFS-LTP is 
      unclear. First, we found that BDNF-LTP, like HFS-LTP, is associated with 
      enhancement in both synaptic strength and granule cell excitability (EPSP-spike 
      coupling). Second, treatment with a competitive NMDA receptor (NMDAR) antagonist 
      blocked HFS-LTP but had no effect on the development or magnitude of BDNF-LTP. 
      Thus, NMDAR activation is not required for the induction or expression of 
      BDNF-LTP. Formation of stable, late phase HFS-LTP requires mRNA synthesis and is 
      coupled to upregulation of the immediate early gene activity-regulated 
      cytoskeleton-associated protein (Arc). Local infusion of the transcription 
      inhibitor actinomycin D (ACD) 1 hr before or immediately before BDNF infusion 
      inhibited BDNF-LTP and upregulation of Arc protein expression. ACD applied 2 hr 
      after BDNF infusion had no effect, defining a critical time window of 
      transcription-dependent synaptic strengthening. Finally, the functional role of 
      BDNF-LTP was assessed in occlusion experiments with HFS-LTP. HFS-LTP was induced, 
      and BDNF was infused at time points corresponding to early phase (1 hr) or late 
      phase (4 hr) HFS-LTP. BDNF applied during the early phase led to normal BDNF-LTP. 
      In contrast, BDNF-LTP was completely occluded during the late phase. The results 
      strongly support a role for BDNF in triggering transcription-dependent, late 
      phase LTP in the intact adult brain.
FAU - Messaoudi, Elhoucine
AU  - Messaoudi E
AD  - Department of Physiology and Locus on Neuroscience, University of Bergen, N-5009 
      Bergen, Norway.
FAU - Ying, Shui-Wang
AU  - Ying SW
FAU - Kanhema, Tambudzai
AU  - Kanhema T
FAU - Croll, Susan D
AU  - Croll SD
FAU - Bramham, Clive R
AU  - Bramham CR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 0 (activity regulated cytoskeletal-associated protein)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacokinetics/*pharmacology
MH  - Cytoskeletal Proteins
MH  - Drug Administration Routes
MH  - Electric Stimulation/methods
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects/physiology
MH  - Hippocampus/*drug effects/physiology
MH  - Immediate-Early Proteins/metabolism
MH  - Long-Term Potentiation/*drug effects/physiology
MH  - *Nerve Tissue Proteins
MH  - Neuronal Plasticity/drug effects/physiology
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
MH  - Synaptic Transmission/drug effects/physiology
MH  - Tissue Distribution
MH  - Transcription, Genetic/*drug effects/physiology
MH  - Up-Regulation/drug effects
PMC - PMC6757978
EDAT- 2002/08/28 10:00
MHDA- 2002/09/14 10:01
PMCR- 2003/03/01
CRDT- 2002/08/28 10:00
PHST- 2002/08/28 10:00 [pubmed]
PHST- 2002/09/14 10:01 [medline]
PHST- 2002/08/28 10:00 [entrez]
PHST- 2003/03/01 00:00 [pmc-release]
AID - 22/17/7453 [pii]
AID - 6728 [pii]
AID - 10.1523/JNEUROSCI.22-17-07453.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Sep 1;22(17):7453-61. doi: 10.1523/JNEUROSCI.22-17-07453.2002.