PMID- 12198406
OWN - NLM
STAT- MEDLINE
DCOM- 20030203
LR  - 20131121
IS  - 0145-6008 (Print)
IS  - 0145-6008 (Linking)
VI  - 26
IP  - 8
DP  - 2002 Aug
TI  - Effects of ethanol and transforming growth factor beta (TGF beta) on neuronal 
      proliferation and nCAM expression.
PG  - 1281-5
AB  - BACKGROUND Developmental events targeted by ethanol are cell proliferation, 
      neuronal migration, and neurite outgrowth; the latter processes being mediated by 
      neural cell adhesion molecule (nCAM). TGFbeta1 affects all three of these events. 
      Therefore, the effects of ethanol on transforming growth factor (TGF) beta1 
      mediated activities in neocortical neurons in vitro were examined. METHODS 
      Primary cultures of cortical neurons were obtained from 16-day-old fetuses and 
      were treated with TGFbeta1 (0 or 10 ng/ml) and ethanol (0 or 400 mg/dl) for 48 
      hr. The effects of these substances on cell numbers, [ H]thymidine incorporation, 
      and the expression of nCAM were determined. RESULTS Both cell growth (the change 
      in cell numbers over time) and cell proliferation were inhibited by TGFbeta1 and 
      ethanol. The action of these two anti-mitogenic factors was additive. In 
      contrast, TGFbeta1 also promoted the expression of three isoforms of nCAM. 
      Likewise, ethanol also up-regulated nCAM expression. On the other hand, ethanol 
      blocked TGFbeta1-mediated nCAM expression, particularly of the 120 and 180 kDa 
      isoforms. CONCLUSIONS TGFbeta ligands inhibit neuronal proliferation and 
      stimulate the expression of cell adhesion proteins that promote the movement of 
      postmitotic neurons and process outgrowth. Ethanol alters these phenomena as 
      well. Thus, in neurons, as in astrocytes, TGFbeta1 and ethanol may interact.
FAU - Miller, Michael W
AU  - Miller MW
AD  - Department of Neuroscience and Physiology, State University of New York-Upstate 
      Medical University, 750 East Adams Street, Syracuse, NY 13210, USA. 
      millermw@upstate.edu
FAU - Luo, Jia
AU  - Luo J
LA  - eng
GR  - AA 06916/AA/NIAAA NIH HHS/United States
GR  - AA 07568/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Alcohol Clin Exp Res
JT  - Alcoholism, clinical and experimental research
JID - 7707242
RN  - 0 (Growth Inhibitors)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Protein Isoforms)
RN  - 0 (Transforming Growth Factor beta)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects/physiology
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/drug effects/metabolism
MH  - Embryo, Mammalian
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Growth Inhibitors/*pharmacology/*physiology
MH  - Neural Cell Adhesion Molecules/*biosynthesis
MH  - Neurons/cytology/*drug effects/metabolism
MH  - Pregnancy
MH  - Protein Isoforms/biosynthesis
MH  - Rats
MH  - Transforming Growth Factor beta/pharmacology/*physiology
MH  - Up-Regulation/drug effects
EDAT- 2002/08/29 10:00
MHDA- 2003/02/04 04:00
CRDT- 2002/08/29 10:00
PHST- 2002/08/29 10:00 [pubmed]
PHST- 2003/02/04 04:00 [medline]
PHST- 2002/08/29 10:00 [entrez]
AID - 10.1097/01.ALC.0000026836.38681.58 [doi]
PST - ppublish
SO  - Alcohol Clin Exp Res. 2002 Aug;26(8):1281-5. doi: 
      10.1097/01.ALC.0000026836.38681.58.