PMID- 12198419
OWN - NLM
STAT- MEDLINE
DCOM- 20020917
LR  - 20150624
IS  - 0032-1052 (Print)
IS  - 0032-1052 (Linking)
VI  - 110
IP  - 4
DP  - 2002 Sep 15
TI  - Simultaneous GDNF and BDNF application leads to increased motoneuron survival and 
      improved functional outcome in an experimental model for obstetric brachial 
      plexus lesions.
PG  - 1066-72
AB  - Motoneurons of the neonate rat respond to proximal axonal injury with morphologic 
      and functional changes and ultimately with neuronal death. Recent studies showed 
      that both glial cell-line-derived neurotrophic factor (GDNF) and brain-derived 
      neurotrophic factor (BDNF) reduce induced degeneration of motoneurons after 
      axotomy and avulsion. Whether rescued motoneurons are functionally intact has 
      been argued. In the present investigation, the authors have used a proximal crush 
      lesion of the brachial plexus in neonatal rats as the experimental model of 
      neuronal injury. This allowed the authors to study the effects of trophic factor 
      administration on injured motoneurons and the relationship between motoneuron 
      survival and extremity function. Trophic factors were locally released by small 
      polymer implants in a low-dose slow-release mode. Six groups of 10 animals were 
      prepared: BDNF, GDNF, GDNF/BDNF, control, sham, and normals. The number of 
      surviving motoneurons was determined by retrograde tracer techniques using 
      Fluorogold and Fastblue. Extremity function was quantitatively evaluated with 
      functional muscle testing at day 56. The results of this study demonstrate that 
      trophic factors applied separately had no effect, whereas combined trophic factor 
      application (GDNF/BDNF group) had a dramatic rescue effect on motoneuron survival 
      as compared with the control groups, which also effected significantly greater 
      strength. The authors conclude that a combination of trophic factors leads to 
      enhanced motoneuron survival, with improved voluntary function as the animal 
      enters adulthood so that exogenous trophic support of motoneurons might have a 
      role in the treatment of all types of severe neonatal plexopathies, maintaining 
      the viability of motoneurons until reconstructive surgery provides them with a 
      pathway for regeneration and endogenous trophic support.
FAU - Aszmann, Oskar C
AU  - Aszmann OC
AD  - Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical 
      School, University of Vienna, Vienna, Austria. oskar.aszmann@univie.ac.at
FAU - Korak, Klaus J
AU  - Korak KJ
FAU - Kropf, Nina
AU  - Kropf N
FAU - Fine, Eric
AU  - Fine E
FAU - Aebischer, Patrick
AU  - Aebischer P
FAU - Frey, Manfred
AU  - Frey M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brachial Plexus Neuropathies/*drug therapy/physiopathology
MH  - Brain-Derived Neurotrophic Factor/administration & 
      dosage/pharmacology/*therapeutic use
MH  - Disease Models, Animal
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Motor Neurons/*drug effects
MH  - Nerve Growth Factors/administration & dosage/pharmacology/*therapeutic use
MH  - Nerve Tissue Proteins/administration & dosage/pharmacology/*therapeutic use
MH  - Paralysis, Obstetric
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/08/29 10:00
MHDA- 2002/09/18 10:01
CRDT- 2002/08/29 10:00
PHST- 2002/08/29 10:00 [pubmed]
PHST- 2002/09/18 10:01 [medline]
PHST- 2002/08/29 10:00 [entrez]
AID - 10.1097/01.PRS.0000020990.82332.43 [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2002 Sep 15;110(4):1066-72. doi: 
      10.1097/01.PRS.0000020990.82332.43.