PMID- 12199582
OWN - NLM
STAT- MEDLINE
DCOM- 20030515
LR  - 20220317
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 74
IP  - 16
DP  - 2002 Aug 15
TI  - Surface modification of poly(dimethylsiloxane) microfluidic devices by 
      ultraviolet polymer grafting.
PG  - 4117-23
AB  - Poly(dimethylsiloxane) (PDMS)-based microfluidic devices are increasing in 
      popularity due to their ease of fabrication and low costs. Despite this, there is 
      a tremendous need for strategies to rapidly and easily tailor the surface 
      properties of these devices. We demonstrate a one-step procedure to covalently 
      link polymers to the surface of PDMS microchannels by ultraviolet graft 
      polymerization. Acrylic acid, acrylamide, dimethylacrylamide, 2-hydroxylethyl 
      acrylate, and poly(ethylene glycol)monomethoxyl acrylate were grafted onto PDMS 
      to yield hydrophilic surfaces. Water droplets possessed contact angles as low as 
      45 degrees on the grafted surfaces. Microchannels constructed from the grafted 
      PDMS were readily filled with aqueous solutions in contrast to devices composed 
      of native PDMS. The grafted surfaces also displayed a substantially reduced 
      adsorption of two test peptides compared to that of oxidized PDMS. Microchannels 
      with grafted surfaces exhibited electroosmotic mobilities intermediate to those 
      displayed by native and oxidized PDMS. Unlike the electroosmotic mobility of 
      oxidized PDMS, the electroosmotic mobility of the grafted surfaces remained 
      stable upon exposure to air. The electrophoretic resolution of two test peptides 
      in the grafted microchannels was considerably improved compared to that in 
      microchannels composed of oxidized PDMS. By using the appropriate monomer, it 
      should be possible to use UV grafting to impart a variety of surface properties 
      to PDMS microfluidics devices.
FAU - Hu, Shuwen
AU  - Hu S
AD  - Center for Biomedical Engineering, University of California, Irvine 92697, USA.
FAU - Ren, Xueqin
AU  - Ren X
FAU - Bachman, Mark
AU  - Bachman M
FAU - Sims, Christopher E
AU  - Sims CE
FAU - Li, G P
AU  - Li GP
FAU - Allbritton, Nancy
AU  - Allbritton N
LA  - eng
GR  - CA78858/CA/NCI NIH HHS/United States
GR  - GM57015/GM/NIGMS NIH HHS/United States
GR  - RR/CA14892/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Dimethylpolysiloxanes/*chemistry/radiation effects
MH  - Electrophoresis, Capillary/*instrumentation
MH  - Equipment Design
MH  - Miniaturization
MH  - Peptides/analysis
MH  - Polymers/chemistry/radiation effects
MH  - Silicones/*chemistry/radiation effects
MH  - Ultraviolet Rays
EDAT- 2002/08/30 10:00
MHDA- 2003/05/16 05:00
CRDT- 2002/08/30 10:00
PHST- 2002/08/30 10:00 [pubmed]
PHST- 2003/05/16 05:00 [medline]
PHST- 2002/08/30 10:00 [entrez]
AID - 10.1021/ac025700w [doi]
PST - ppublish
SO  - Anal Chem. 2002 Aug 15;74(16):4117-23. doi: 10.1021/ac025700w.