PMID- 12199774
OWN - NLM
STAT- MEDLINE
DCOM- 20021118
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 118
IP  - 4
DP  - 2002 Sep
TI  - CD40L stimulation enhances the ability of conventional metaphase cytogenetics to 
      detect chromosome aberrations in B-cell chronic lymphocytic leukaemia cells.
PG  - 968-75
AB  - Conventional metaphase cytogenetics underestimates the frequency of specific 
      chromosome aberrations in B-cell chronic lymphocytic leukaemia (B-CLL) as a 
      result of the very low proliferative activity of these cells in vitro. New 
      molecular approaches, such as fluorescence in situ hybridization (FISH) or 
      comparative genomic hybridization (CGH), may circumvent this problem, at least in 
      part, but these techniques are either strongly dependent on the knowledge of 
      candidate regions or detect only unbalanced aberrations. In the present study, we 
      analysed 27 B-CLL peripheral blood samples by metaphase cytogenetics after CD40 
      ligand (CD40L)-induced cell cycle stimulation. In comparison with the 
      simultaneous use of B-cell mitogens such as 12-O-tetradecanoylphorbol-13-acetate 
      (TPA), lipopolysaccharide (LPS) and pokeweed mitogen (PWM), CD40L stimulation of 
      B-CLL cells induced a threefold increase in metaphases amenable to analysis by 
      conventional cytogenetics. The analysis of these metaphases confirmed all genetic 
      abnormalities detected by FISH. Moreover, CD40L-enhanced cytogenetics revealed 
      complex karyotypic aberrations in 11 out of 27 patients (41%). In one case, a 
      balanced translocation t(11;16)(p15;p13.1), so far unreported in B-CLL, was 
      detected. Taken together, the results of our study show the potential of 
      CD40L-enhanced metaphase cytogenetics to detect more and new chromosome 
      aberrations in B-CLL.
FAU - Buhmann, Raymund
AU  - Buhmann R
AD  - GSF, National Research Center for Environment and Health, Genzentrum and 
      Medizinische Klinik III, Klinikum der Universitaet Muenchen Grosshadern, Germany. 
      buhmann@lmb.uni-muenchen.de
FAU - Kurzeder, Christian
AU  - Kurzeder C
FAU - Rehklau, Jutta
AU  - Rehklau J
FAU - Westhaus, Doreen
AU  - Westhaus D
FAU - Bursch, Sabina
AU  - Bursch S
FAU - Hiddemann, Wolfgang
AU  - Hiddemann W
FAU - Haferlach, Torsten
AU  - Haferlach T
FAU - Hallek, Michael
AU  - Hallek M
FAU - Schoch, Claudia
AU  - Schoch C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - 3T3 Cells
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - B-Lymphocytes/pathology
MH  - CD40 Ligand/*pharmacology
MH  - Cell Cycle/drug effects
MH  - *Chromosome Aberrations
MH  - Coculture Techniques
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/immunology
MH  - Male
MH  - Metaphase
MH  - Mice
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Transfection
EDAT- 2002/08/30 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/08/30 10:00
PHST- 2002/08/30 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/08/30 10:00 [entrez]
AID - 3719 [pii]
AID - 10.1046/j.1365-2141.2002.03719.x [doi]
PST - ppublish
SO  - Br J Haematol. 2002 Sep;118(4):968-75. doi: 10.1046/j.1365-2141.2002.03719.x.