PMID- 12200318
OWN - NLM
STAT- MEDLINE
DCOM- 20021011
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 68
IP  - 9
DP  - 2002 Sep
TI  - Microscopic examination of distribution and phenotypic properties of 
      phylogenetically diverse Chloroflexaceae-related bacteria in hot spring microbial 
      mats.
PG  - 4593-603
AB  - We investigated the diversity, distribution, and phenotypes of uncultivated 
      Chloroflexaceae-related bacteria in photosynthetic microbial mats of an alkaline 
      hot spring (Mushroom Spring, Yellowstone National Park). By applying a directed 
      PCR approach, molecular cloning, and sequence analysis of 16S rRNA genes, an 
      unexpectedly large phylogenetic diversity among these bacteria was detected. 
      Oligonucleotide probes were designed to target 16S rRNAs from organisms 
      affiliated with the genus Chloroflexus or with the type C cluster, a group of 
      previously discovered Chloroflexaceae relatives of this mat community. The 
      application of peroxidase-labeled probes in conjunction with tyramide signal 
      amplification enabled the identification of these organisms within the microbial 
      mats by fluorescence in situ hybridization (FISH) and the investigation of their 
      morphology, abundance, and small-scale distribution. FISH was combined with 
      oxygen microelectrode measurements, microscope spectrometry, and 
      microautoradiography to examine their microenvironment, pigmentation, and carbon 
      source usage. Abundant type C-related, filamentous bacteria were found to 
      flourish within the cyanobacterium-dominated, highly oxygenated top layers and to 
      predominate numerically in deeper orange-colored zones of the investigated 
      microbial mats, correlating with the distribution of bacteriochlorophyll a. 
      Chloroflexus sp. filaments were rare at 60 degrees C but were more abundant at 70 
      degrees C, where they were confined to the upper millimeter of the mat. Both type 
      C organisms and Chloroflexus spp. were observed to assimilate radiolabeled 
      acetate under in situ conditions.
FAU - Nubel, Ulrich
AU  - Nubel U
AD  - Department of Land Resources and Environmental Sciences, Montana State 
      University, Bozeman, Montana, USA. unuebel@dsmz.de
FAU - Bateson, Mary M
AU  - Bateson MM
FAU - Vandieken, Verona
AU  - Vandieken V
FAU - Wieland, Andrea
AU  - Wieland A
FAU - Kuhl, Michael
AU  - Kuhl M
FAU - Ward, David M
AU  - Ward DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Pigments, Biological)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Autoradiography
MH  - Chlorobi/*classification/genetics
MH  - Cyanobacteria/physiology
MH  - Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy
MH  - Oxygen/metabolism
MH  - Phenotype
MH  - Phylogeny
MH  - Pigments, Biological/metabolism
MH  - Temperature
MH  - *Water Microbiology
PMC - PMC124081
EDAT- 2002/08/30 10:00
MHDA- 2002/10/12 04:00
PMCR- 2002/09/01
CRDT- 2002/08/30 10:00
PHST- 2002/08/30 10:00 [pubmed]
PHST- 2002/10/12 04:00 [medline]
PHST- 2002/08/30 10:00 [entrez]
PHST- 2002/09/01 00:00 [pmc-release]
AID - 0254 [pii]
AID - 10.1128/AEM.68.9.4593-4603.2002 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2002 Sep;68(9):4593-603. doi: 
      10.1128/AEM.68.9.4593-4603.2002.