PMID- 12202124
OWN - NLM
STAT- MEDLINE
DCOM- 20021106
LR  - 20191106
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 10
IP  - 9
DP  - 2002 Sep
TI  - Biomarkers of joint tissue metabolism in canine osteoarthritic and arthritic 
      joint disorders.
PG  - 714-21
AB  - OBJECTIVE: To explore the levels of matrix metalloprotease-3 (MMP-3), tissue 
      inhibitor of metalloproteases-1 (TIMP-1), 5D4 keratan sulfate, and two 3B3 
      chondroitin-sulfate epitopes in several canine osteoarthritic and inflammatory 
      arthropathies. METHODS: Blood and synovial fluid were obtained from 103 dogs with 
      rupture of the anterior cruciate ligament (ACLR), osteochondritis dissecans 
      (OCD), fragmented coronoid process (FPC), patella luxation (PL), hip dysplasia 
      (HD) or infectious arthritis. Dogs with non-musculosceletal disorders were used 
      as controls. The biomarkers were measured by immunoassays. RESULTS: Median levels 
      of synovial MMP-3, TIMP-1 and molar ratios of MMP/TIMP-1 were significantly 
      higher in the arthritis than in the control group. The release of 5D4 keratan 
      sulfate epitope and serum 3B3 neoepitope was reduced in arthritis patients. 
      Increases in synovial TIMP-1 in OA were less pronounced and the molar ratio of 
      MMP-3/TIMP-1 remained far below 1.0, demonstrating a surplus of the protease 
      inhibitor. In osteoarthritic patients median levels of synovial 5D4 keratan 
      sulfate were up-regulated after ACLR and PL and were inversely correlated with 
      increasing duration of lameness. Serum TIMP-1 levels were significantly reduced 
      in the joint disorder group when compared with the control group. CONCLUSION: Our 
      observations present the TIMP-1 serum level as a potential marker for the 
      detection of degenerative changes in cartilage and also indicate that in canine 
      OA, the MMP-3 mediated matrix destruction is not of major importance. However 
      MMP-3 seems to be a sensitive marker for the local inflammation in canine 
      arthritis.
CI  - Copyright 2002 OsteoArthritis Research Society International. Published by 
      Elsevier Science Ltd. All rights reserved.
FAU - Hegemann, N
AU  - Hegemann N
AD  - Institute of Immunology and Molecular Biology, Faculty of Veterinary Medicine, 
      Free University of Berlin, Germany
FAU - Kohn, B
AU  - Kohn B
FAU - Brunnberg, L
AU  - Brunnberg L
FAU - Schmidt, M F
AU  - Schmidt MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (Epitopes)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 9007-28-7 (Chondroitin Sulfates)
RN  - 9056-36-4 (Keratan Sulfate)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament Injuries
MH  - Arthritis, Infectious/blood
MH  - Chondroitin Sulfates/*analysis/blood
MH  - Dogs
MH  - Epitopes/analysis/blood
MH  - Hip Dysplasia, Canine/blood
MH  - Keratan Sulfate/*analysis/blood
MH  - Matrix Metalloproteinase 3/*analysis/blood
MH  - Osteoarthritis/*blood/diagnosis
MH  - Osteochondritis Dissecans/blood
MH  - Patella/injuries
MH  - Rupture/blood
MH  - Tissue Inhibitor of Metalloproteinase-1/*analysis/blood
MH  - Ulna Fractures/blood
EDAT- 2002/08/31 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/08/31 10:00
PHST- 2002/08/31 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/08/31 10:00 [entrez]
AID - S1063458402908204 [pii]
AID - 10.1053/joca.2002.0820 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2002 Sep;10(9):714-21. doi: 10.1053/joca.2002.0820.