PMID- 12202482
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20220310
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 45
DP  - 2002 Nov 8
TI  - Regulation of TRKB surface expression by brain-derived neurotrophic factor and 
      truncated TRKB isoforms.
PG  - 43160-7
AB  - Brain-derived neurotrophic factor (BDNF) signaling through its receptor TRKB 
      modulates survival, differentiation, and activity of neurons. BDNF activates TRKB 
      on the cell surface, which leads to the initiation of intracellular signaling 
      cascades and different biological responses in neurons. Neuronal activity has 
      been shown to regulate TRKB levels on the plasma membrane of neurons, but little 
      is known about other factors affecting TRKB surface expression levels. We report 
      here that BDNF regulates the cell surface levels of transfected or endogenously 
      expressed full-length TRKB, depending on the exposure time in neuroblastoma cells 
      and primary hippocampal neurons. BDNF rapidly increases TRKB surface expression 
      levels in seconds, whereas treatment of cells with BDNF for a longer time 
      (minutes to hours) leads to decreased TRKB surface levels. Coexpression of the 
      full-length TRKB together with the truncated TRKB.T1 isoform results in decreased 
      levels of full-length TRKB on the cell surface. This effect is specific to the T1 
      isoform, because coexpression of a kinase-dead TRKB mutant or another kinase 
      domain-lacking TRKB form, truncated T-Shc, leads to increased TRKB surface 
      levels. Our results suggest that regulation of TRKB surface expression levels by 
      different factors is tightly controlled by complex mechanisms in active neurons.
FAU - Haapasalo, Annakaisa
AU  - Haapasalo A
AD  - Department of Neurobiology, University of Kuopio, P. O. Box 1627, 70211 Kuopio, 
      Finland.
FAU - Sipola, Ilkka
AU  - Sipola I
FAU - Larsson, Kim
AU  - Larsson K
FAU - Akerman, Karl E O
AU  - Akerman KE
FAU - Stoilov, Peter
AU  - Stoilov P
FAU - Stamm, Stefan
AU  - Stamm S
FAU - Wong, Garry
AU  - Wong G
FAU - Castren, Eero
AU  - Castren E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020828
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA Primers)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Membrane/*physiology
MH  - DNA Primers
MH  - *Gene Expression Regulation/drug effects
MH  - Hippocampus/physiology
MH  - Kinetics
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Neuroblastoma
MH  - Neurons/drug effects/*physiology
MH  - Point Mutation
MH  - Protein Isoforms/genetics/pharmacology
MH  - Receptor, trkB/*genetics
MH  - Recombinant Proteins
MH  - Sequence Deletion
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2002/08/31 10:00
MHDA- 2003/02/07 04:00
CRDT- 2002/08/31 10:00
PHST- 2002/08/31 10:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/08/31 10:00 [entrez]
AID - S0021-9258(19)71918-8 [pii]
AID - 10.1074/jbc.M205202200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Nov 8;277(45):43160-7. doi: 10.1074/jbc.M205202200. Epub 2002 
      Aug 28.