PMID- 12202574
OWN - NLM
STAT- MEDLINE
DCOM- 20021114
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 40
IP  - 9
DP  - 2002 Sep
TI  - Possible connection between a widely disseminated conjugative gentamicin 
      resistance (pMG1-like) plasmid and the emergence of vancomycin resistance in 
      Enterococcus faecium.
PG  - 3326-33
AB  - A total of 640 vancomycin-resistant Enterococcus faecium (VRE) isolates, which 
      were obtained between 1994 and 1999 from the Medical School Hospital of the 
      University of Michigan, Ann Arbor, were used in this study. Of the 640 strains, 
      611 and 29 were VanA and VanB VRE, respectively, based on PCR analysis. Four 
      hundred ninety-two (77%) of the strains exhibited resistance to concentrations of 
      gentamicin from 64 micro g/ml (MIC) to more than 1,024 micro g/ml (MIC). The 
      gentamicin resistance of each of 261 (53%) of the 492 gentamicin-resistant 
      strains was transferred to E. faecium at a frequency of about 10(-5) to 10(-6) 
      per donor cell in broth mating. More than 90% of vancomycin resistances of the 
      261 strains cotransferred with the gentamicin resistances to E. faecium strains 
      by filter mating. The conjugative gentamicin resistance plasmids were identified 
      and were classified into five types (A through E) with respect to their EcoRI 
      restriction profiles. The transfer frequencies of each type of plasmid between E. 
      faecium strains or Enterococcus faecalis strains were around 10(-3) to 10(-5) per 
      donor cell or around 10(-6) to 10(-7) per donor cell, respectively, in broth 
      mating. Type A and type B were the most frequently isolated, at an isolation 
      frequency of about 40% per VRE isolate harboring the gentamicin resistance 
      conjugative plasmid. The plasmids did not show any homology in Southern 
      hybridization with the pheromone-responsive plasmids and broad-host-range 
      plasmids pAMbeta1 and pIP501. The EcoRI or NdeI restriction fragments of each 
      type of plasmids hybridized to the conjugative gentamicin resistance plasmid pMG1 
      (65.1 kb), which was originally isolated from an E. faecium clinical isolate, and 
      transfer efficiently in broth mating.
FAU - Tomita, Haruyoshi
AU  - Tomita H
AD  - Department of Microbiology, Gunma University School of Medicine, Maebashi, Gunma 
      371-8511, Japan.
FAU - Pierson, Carl
AU  - Pierson C
FAU - Lim, Suk Kyung
AU  - Lim SK
FAU - Clewell, Don B
AU  - Clewell DB
FAU - Ike, Yasuyoshi
AU  - Ike Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Culture Media)
RN  - 0 (Gentamicins)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - *Conjugation, Genetic
MH  - Culture Media
MH  - Drug Resistance, Bacterial/genetics
MH  - Enterococcus faecium/*drug effects/genetics
MH  - Gene Transfer, Horizontal
MH  - Gentamicins/*pharmacology
MH  - Nucleic Acid Hybridization
MH  - Plasmids/*genetics
MH  - *Vancomycin Resistance
PMC - PMC130708
EDAT- 2002/08/31 10:00
MHDA- 2002/11/26 04:00
PMCR- 2002/09/01
CRDT- 2002/08/31 10:00
PHST- 2002/08/31 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/08/31 10:00 [entrez]
PHST- 2002/09/01 00:00 [pmc-release]
AID - 0188 [pii]
AID - 10.1128/JCM.40.9.3326-3333.2002 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2002 Sep;40(9):3326-33. doi: 10.1128/JCM.40.9.3326-3333.2002.