PMID- 12203788
OWN - NLM
STAT- MEDLINE
DCOM- 20021203
LR  - 20091119
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 35
IP  - 1
DP  - 2002 Sep
TI  - Hemizygous deletions in the HLA region account for loss of heterozygosity in the 
      majority of diffuse large B-cell lymphomas of the testis and the central nervous 
      system.
PG  - 38-48
AB  - Loss of heterozygosity (LOH) is a major mechanism for inactivation of 
      tumor-suppressor genes and has been observed in various solid tumors and 
      lymphomas. The human leukocyte antigen (HLA) region is located at chromosome band 
      6p21.3, and loss or alteration of this region may provide tumor cells with a 
      mechanism to escape from the immune system. We previously identified small 
      homozygous deletions within the HLA class II region in many of the diffuse large 
      B-cell lymphomas (DLCLs) of the central nervous system (CNS) and the testis. In 
      the present study, we focused on the mechanism leading to LOH in the HLA region. 
      Twenty microsatellite markers, of which 12 were specific for HLA, were applied on 
      11 extranodal DLCLs of the CNS and 28 of the testis. Additionally, fluorescence 
      in situ hybridization with seven HLA-specific probes and a centromere 6-specific 
      probe was performed on 20 cases to study the mechanism of LOH. In contrast to 
      previously published data on spontaneously mutated lymphoblastoid cell lines, 
      intrachromosomal hemizygous deletion, not mitotic recombination, was the major 
      cause of LOH of the HLA region in these lymphomas. However, opposed to data in 
      colorectal cancer, these deletions were rarely (one of nine cases) associated 
      with an interchromosomal rearrangement such as a translocation.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Jordanova, Ekaterina S
AU  - Jordanova ES
AD  - Department of Pathology, Leiden University Medical Center, Leiden, The 
      Netherlands. k.jordanova@lumc.nl
FAU - Riemersma, Sietske A
AU  - Riemersma SA
FAU - Philippo, Katja
AU  - Philippo K
FAU - Giphart-Gassler, Micheline
AU  - Giphart-Gassler M
FAU - Schuuring, Ed
AU  - Schuuring E
FAU - Kluin, Philip M
AU  - Kluin PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Alleles
MH  - *Chromosome Deletion
MH  - Chromosome Inversion
MH  - Chromosome Segregation/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - DNA Probes/genetics
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase/genetics
MH  - Loss of Heterozygosity/*genetics
MH  - Lymphoma, B-Cell/*genetics
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics
MH  - Male
MH  - Mitosis/genetics
MH  - Monosomy/genetics
MH  - Recombination, Genetic/genetics
MH  - Testicular Neoplasms/*genetics
MH  - Translocation, Genetic
EDAT- 2002/08/31 10:00
MHDA- 2002/12/04 04:00
CRDT- 2002/08/31 10:00
PHST- 2002/08/31 10:00 [pubmed]
PHST- 2002/12/04 04:00 [medline]
PHST- 2002/08/31 10:00 [entrez]
AID - 10.1002/gcc.10093 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2002 Sep;35(1):38-48. doi: 10.1002/gcc.10093.