PMID- 12204226
OWN - NLM
STAT- MEDLINE
DCOM- 20021121
LR  - 20190826
IS  - 0020-7519 (Print)
IS  - 0020-7519 (Linking)
VI  - 32
IP  - 10
DP  - 2002 Sep
TI  - Strain typing in Leishmania donovani by using sequence-confirmed amplified region 
      analysis.
PG  - 1267-76
AB  - To differentiate strains of Leishmania donovani, allelic markers at the DNA level 
      were developed by sequence-confirmed amplified region analysis (SCAR). Homologous 
      fragments from different strains of L. donovani were amplified by PCR using 
      random primers and subsequently screened for single-strand conformation 
      polymorphisms. Direct sequencing revealed 55 sequence polymorphisms in eight 
      co-dominant DNA markers; 38 of them were single point mutations. Heterozygosity 
      was evident for 69% and fixed heterozygosity for 25% of all polymorphisms. At 
      most polymorphic sites one of the segregation genotypes was missing. Nineteen 
      unique multilocus genotypes were identified among 29 strains of L. donovani. One 
      genotype was represented by eight Sudanese strains; also two strains from Sudan 
      as well as two strains from Kenya, respectively, shared identical genotypes. All 
      other strains had individual multilocus genotypes. Calculation of genetic 
      distances showed a correlation between multilocus genotypes and the geographical 
      origin of these strains. African strains were found in one well-supported cluster 
      with Kenyan and Sudanese strains clearly separated. SCAR markers seem to 
      represent a random sample of neutral genetic variation present in natural 
      populations. They are co-dominant because they can detect all possible allele 
      combinations in a diploid organism and may, therefore, be very useful for 
      population genetic analysis in Leishmania.
FAU - Lewin, Sylke
AU  - Lewin S
AD  - Institute of Microbiology and Hygiene, Charite Campus Mitte, Dorotheenstrasse 96, 
      D-10117 Berlin, Germany.
FAU - Schonian, Gabriele
AU  - Schonian G
FAU - El Tai, Nahla
AU  - El Tai N
FAU - Oskam, Linda
AU  - Oskam L
FAU - Bastien, Patrick
AU  - Bastien P
FAU - Presber, Wolfgang
AU  - Presber W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (DNA, Protozoan)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - DNA, Protozoan/*analysis
MH  - Genotype
MH  - Leishmania donovani/*classification/genetics/isolation & purification
MH  - Leishmaniasis/epidemiology/immunology
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Phylogeny
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA
EDAT- 2002/09/03 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/09/03 10:00
PHST- 2002/09/03 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/09/03 10:00 [entrez]
AID - S0020751902000917 [pii]
AID - 10.1016/s0020-7519(02)00091-7 [doi]
PST - ppublish
SO  - Int J Parasitol. 2002 Sep;32(10):1267-76. doi: 10.1016/s0020-7519(02)00091-7.