PMID- 12204585
OWN - NLM
STAT- MEDLINE
DCOM- 20021016
LR  - 20191106
IS  - 0166-445X (Print)
IS  - 0166-445X (Linking)
VI  - 60
IP  - 1-2
DP  - 2002 Oct 2
TI  - Physiological and cytological responses of the marine diatom Skeletonema costatum 
      to 2,4-dichlorophenol.
PG  - 33-41
AB  - Physiological and cytological responses of the marine diatom, Skeletonema 
      costatum, to 2,4-dichlorophenol (2,4-DCP) were investigated after exposing algal 
      cultures to 1-6 mg x l(-1) of 2,4-DCP for 96 h. Growth rate of the diatom was 
      significantly reduced by 2,4-DCP at and above 3.0 mg x l(-1) while other 
      biological parameters such as photosynthetic and respiration rates, carotenoid 
      and protein content, ATP level and adenylate energy charge were unaffected. 
      Exposure to 6.0 mg x l(-1) 2,4-DCP for 96 h resulted in the total lipid content 
      being increased to 304%, while the RNA/DNA ratio was reduced to 31% of the 
      control values. A parallel study by transmission electron microscopy further 
      confirmed the increase in cellular lipid content, as evidenced by the 
      accumulation of lipid droplets within diatom cells. A slight increase in 
      carbohydrate (+37.8%) and decrease in chlorophyll a (-20.4%) and total 
      chlorophyll c (-14.4%) were also found at 6.0 mg x l(-1) 2,4-DCP. Although 
      2,4-DCP is known to uncouple oxidative phosphorylation, our results show that 
      energy production was not significantly inhibited at sublethal concentrations of 
      2,4-DCP. The observed growth inhibition in S. costatum caused by 2,4-DCP was 
      associated with an increase in energy storage and inhibition of protein 
      synthesis, as indicated by a reduction in RNA/DNA ratio.
FAU - Yang, Shao
AU  - Yang S
AD  - Department of Biology and Chemistry and Centre for Coastal Pollution and 
      Conservation, City University of Hong Kong, Tat Chee Avenue, Kowloon, Special 
      Administrative Region, Hong Kong, China.
FAU - Wu, Rudolf S S
AU  - Wu RS
FAU - Kong, Richard Y C
AU  - Kong RY
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Anthelmintics)
RN  - 0 (Chlorophenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - 63231-63-0 (RNA)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9007-49-2 (DNA)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Adaptation, Physiological
MH  - Adenosine Triphosphate/analysis
MH  - Anthelmintics/*adverse effects
MH  - Chlorophenols/*adverse effects
MH  - DNA/analysis
MH  - Diatoms/*physiology
MH  - Energy Metabolism/*drug effects
MH  - Photosynthesis/drug effects
MH  - Population Dynamics
MH  - Protein Biosynthesis
MH  - RNA/analysis
MH  - Water Pollutants, Chemical/*adverse effects
EDAT- 2002/09/03 10:00
MHDA- 2002/10/17 04:00
CRDT- 2002/09/03 10:00
PHST- 2002/09/03 10:00 [pubmed]
PHST- 2002/10/17 04:00 [medline]
PHST- 2002/09/03 10:00 [entrez]
AID - S0166445X01002582 [pii]
AID - 10.1016/s0166-445x(01)00258-2 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2002 Oct 2;60(1-2):33-41. doi: 10.1016/s0166-445x(01)00258-2.