PMID- 12207590
OWN - NLM
STAT- MEDLINE
DCOM- 20021122
LR  - 20190704
IS  - 0007-0963 (Print)
IS  - 0007-0963 (Linking)
VI  - 147
IP  - 3
DP  - 2002 Sep
TI  - Premature apoptosis of keratinocytes and the dysregulation of keratinization in 
      porokeratosis.
PG  - 498-502
AB  - BACKGROUND: Porokeratosis is a dyskeratotic disorder of the skin characterized by 
      cornoid lamella with parakeratosis, hyperkeratosis and loss of granular layers. 
      The pathogenesis of porokeratosis and the mechanism(s) of its abnormal 
      keratinization are still unknown. OBJECTIVE: To elucidate the mechanism(s) of 
      abnormal keratinization that leads to the formation of cornoid lamellae in 
      porokeratosis. METHODS: Apoptosis of keratinocytes was assessed in the skin of 
      seven patients by an in situ apoptosis assay based on the terminal 
      deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) 
      reaction. Patterns of loricrin and involucrin expression were examined by 
      immunohistochemistry. RESULTS: TUNEL-positive keratinocytes were observed in the 
      epidermis underlying the cornoid lamella in all cases examined. Furthermore, 
      loricrin expression was interrupted there, in contrast to involucrin, which was 
      expressed diffusely in the lesional epidermis. CONCLUSIONS: These results suggest 
      that an abnormal early keratinocyte apoptosis accompanied by dysregulation of 
      terminal differentiation of those cells may be involved in the pathogenesis of 
      porokeratosis.
FAU - Shen, C-S
AU  - Shen CS
AD  - Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Tabata, K
AU  - Tabata K
FAU - Matsuki, M
AU  - Matsuki M
FAU - Goto, T
AU  - Goto T
FAU - Yokochi, T
AU  - Yokochi T
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Dermatol
JT  - The British journal of dermatology
JID - 0004041
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (loricrin)
RN  - 60108-77-2 (involucrin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Apoptosis
MH  - Cell Differentiation
MH  - Down-Regulation
MH  - Epidermis/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Situ Nick-End Labeling
MH  - Keratinocytes/*pathology
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Middle Aged
MH  - Porokeratosis/metabolism/*pathology
MH  - Protein Precursors/metabolism
EDAT- 2002/09/05 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/09/05 10:00
PHST- 2002/09/05 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/09/05 10:00 [entrez]
AID - 4853 [pii]
AID - 10.1046/j.1365-2133.2002.04853.x [doi]
PST - ppublish
SO  - Br J Dermatol. 2002 Sep;147(3):498-502. doi: 10.1046/j.1365-2133.2002.04853.x.