PMID- 12210835
OWN - NLM
STAT- MEDLINE
DCOM- 20021115
LR  - 20071114
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 69
IP  - 5
DP  - 2002 Sep 1
TI  - Human bone marrow stromal cell cultures conditioned by traumatic brain tissue 
      extracts: growth factor production.
PG  - 687-91
AB  - Treatment of traumatic brain injury (TBI) with bone marrow stromal cells (MSCs) 
      improves functional outcome in the rat. However, the specific mechanisms by which 
      introduced MSCs provide benefit remain to be elucidated. Currently, the ability 
      of therapeutically transplanted MSCs to replace injured parenchymal CNS tissue 
      appears limited at best. Tissue replacement, however, is not the only possible 
      compensatory avenue in cell transplantation therapy. Various growth factors have 
      been shown to mediate the repair and replacement of damaged tissue, so trophic 
      support provided by transplanted MSCs may play a role in the treatment of damaged 
      tissue. We therefore investigated the temporal profile of various growth factors, 
      brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular 
      endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and 
      hepatocyte growth factor (HGF), within cultures of human MSCs (hMSCs) conditioned 
      with cerebral tissue extract from TBI. hMSCs were cultured with TBI extracts of 
      rat brain in vitro and quantitative sandwich enzyme-linked immunosorbent assays 
      (ELISAs) were performed. TBI-conditioned hMSCs cultures demonstrated a 
      time-dependent increase of BDNF, NGF, VEGF, and HGF, indicating a responsive 
      production of these growth factors by the hMSCs. The ELISA data suggest that 
      transplanted hMSCs may provide therapeutic benefit via a responsive secretion of 
      an array of growth factors that can foster neuroprotection and angiogenesis.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Chen, Xiaoguang
AU  - Chen X
AD  - Department of Neurology, Henry Ford Health Sciences Center, Detroit, Michigan 
      48202, USA.
FAU - Katakowski, Mark
AU  - Katakowski M
FAU - Li, Yi
AU  - Li Y
FAU - Lu, Dunyue
AU  - Lu D
FAU - Wang, Lei
AU  - Wang L
FAU - Zhang, Lijie
AU  - Zhang L
FAU - Chen, Jieli
AU  - Chen J
FAU - Xu, Yongxian
AU  - Xu Y
FAU - Gautam, Subhash
AU  - Gautam S
FAU - Mahmood, Asim
AU  - Mahmood A
FAU - Chopp, Michael
AU  - Chopp M
LA  - eng
GR  - P01 NS23393/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Lymphokines)
RN  - 0 (Tissue Extracts)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Brain Chemistry
MH  - Brain Injuries/pathology
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - Endothelial Growth Factors/biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibroblast Growth Factor 2/biosynthesis
MH  - Growth Substances/*biosynthesis
MH  - Hepatocyte Growth Factor/biosynthesis
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/biosynthesis
MH  - Lymphokines/biosynthesis
MH  - Male
MH  - Nerve Growth Factor/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/*drug effects/metabolism
MH  - Tissue Extracts/chemistry/*pharmacology
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2002/09/05 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/09/05 10:00
PHST- 2002/09/05 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/09/05 10:00 [entrez]
AID - 10.1002/jnr.10334 [doi]
PST - ppublish
SO  - J Neurosci Res. 2002 Sep 1;69(5):687-91. doi: 10.1002/jnr.10334.