PMID- 12211427
OWN - NLM
STAT- MEDLINE
DCOM- 20030407
LR  - 20141120
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 17
IP  - 9
DP  - 2002 Sep
TI  - Insulin-like growth factor I effect on the number of osteoblast progenitors is 
      impaired in ovariectomized mice.
PG  - 1579-87
AB  - Because insulin-like growth factor (IGF) I is an important regulator of bone 
      formation, we proposed the hypothesis that IGF-I could contribute in regulating 
      the number of osteoblast progenitors (colony-forming unit fibroblast with ALP 
      activity [CFU-F/ALP+]). To test ex vivo and in vivo effects of IGF-I on the 
      number of CFU-F/ALP+, bone marrow cells (BMCs) derived from normal mice, growth 
      hormone (GH)-deficient lit/lit mice, or ovariectomized (OVX) mice were cultured 
      and the CFU-F/ALP+ number was counted. Ex vivo treatment of IGF-I increased the 
      CFU-F/ALP+ number in a dose-dependent manner compared with vehicle-treated 
      control cultures. The CFU-F/ALP+ number was decreased by 20% (p < 0.01; n = 7-9) 
      in GH-deficient lit/lit mice compared with age-matched control mice. Four weeks 
      after OVX or sham operation, IGF-I (2 microg/g body wt) or vehicle was 
      administered twice on day 1, and 5 days later, BMCs were removed from the femur 
      and cultured for 10 days (n = 9-10 per group). IGF-I administration increased the 
      CFU-F/ALP+ number by 63% (p < 0.01) and 19% (NS), respectively, in sham-operated 
      (sham) and OVX mice compared with the vehicle-treated control group. The serum 
      IGF-I level was similar in OVX mice compared with sham mice; this finding is 
      different from that found in rats in which OVX increases the serum IGF-I level. 
      This study showed that IGF-I is an important regulator of osteoblast-progenitor 
      number in the BMCs of mice both ex vivo and in vivo and that the IGF-I response 
      to increase the number of osteoblast progenitors was impaired in OVX mice.
FAU - Kasukawa, Yuji
AU  - Kasukawa Y
AD  - Musculoskeletal Disease Center, JL Pettis Veterans Administration Medical Center, 
      Loma Linda, California 92357, USA.
FAU - Stabnov, Lisa
AU  - Stabnov L
FAU - Miyakoshi, Naohisa
AU  - Miyakoshi N
FAU - Baylink, David J
AU  - Baylink DJ
FAU - Mohan, Subburaman
AU  - Mohan S
LA  - eng
GR  - AR31062/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (Receptors, LHRH)
RN  - 0 (Recombinant Proteins)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Growth Hormone/deficiency
MH  - In Vitro Techniques
MH  - Insulin-Like Growth Factor I/*pharmacology/physiology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Models, Biological
MH  - Osteoblasts/cytology/*drug effects/enzymology
MH  - Ovariectomy
MH  - Receptors, LHRH/genetics
MH  - Recombinant Proteins/pharmacology
MH  - Stem Cells/cytology/drug effects/enzymology
EDAT- 2002/09/05 10:00
MHDA- 2003/04/08 05:00
CRDT- 2002/09/05 10:00
PHST- 2002/09/05 10:00 [pubmed]
PHST- 2003/04/08 05:00 [medline]
PHST- 2002/09/05 10:00 [entrez]
AID - 10.1359/jbmr.2002.17.9.1579 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2002 Sep;17(9):1579-87. doi: 10.1359/jbmr.2002.17.9.1579.