PMID- 12213492
OWN - NLM
STAT- MEDLINE
DCOM- 20021029
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1584
IP  - 1
DP  - 2002 Sep 5
TI  - Molecular characterization of the human PLC beta1 gene.
PG  - 46-54
AB  - Inositide-specific phospholipase C (PLC) signaling constitutes a central 
      intermediate in a number of cellular functions among which the control of cell 
      growth raises a particular interest. Indeed, we have previously shown that 
      nuclear phospholipase C beta1 (PLC beta1) is central for the regulation of 
      mitogen-induced cell growth. We have also assigned by fluorescence in situ 
      hybridization (FISH) analysis the PLC beta1 to human chromosome 20p12. In this 
      study, we have carried out a detailed analysis of the human gene, showing the 
      existence of alternative splicing, which gives rise, besides the two forms (1a 
      and 1b) already shown in rodents, to a new 600 bp smaller form coding for a 110 
      kDa protein. We have also identified a new exon at the 5', showing no homology 
      with the rodent sequence. Here we provide the complete determination of the 
      exon/intron structure of the gene spanning 250 kb of DNA. We found that the exons 
      are quite small, ranging from 49 to 222 bp, while the introns vary between 108 bp 
      and 34,400 bp. The availability of the understanding of the genome organization 
      of this inositide-specific PLC, which represents a key step of the cell cycle 
      related signaling, could actually pave the way for further genetic analysis of 
      p12 region of human chromosome 20 in diseases involving alterations of the 
      control of cell growth such as malignancies.
FAU - Peruzzi, Daniela
AU  - Peruzzi D
AD  - Department of Anatomical Sciences, Cellular Signalling Laboratory, University of 
      Bologna, Via Irnerio, 48, I-40126 Bologna, Italy.
FAU - Aluigi, Michela
AU  - Aluigi M
FAU - Manzoli, Lucia
AU  - Manzoli L
FAU - Billi, Anna Maria
AU  - Billi AM
FAU - Di Giorgio, Francesco Paolo
AU  - Di Giorgio FP
FAU - Morleo, Manuela
AU  - Morleo M
FAU - Martelli, Alberto M
AU  - Martelli AM
FAU - Cocco, Lucio
AU  - Cocco L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Isoenzymes)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCB1 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C beta)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 5' Untranslated Regions
MH  - Adult
MH  - Alternative Splicing
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - DNA, Complementary/biosynthesis/chemistry
MH  - Fetus
MH  - Gene Amplification
MH  - Humans
MH  - Isoenzymes/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Phospholipase C beta
MH  - Promoter Regions, Genetic
MH  - Transfection
MH  - Type C Phospholipases/chemistry/*genetics
EDAT- 2002/09/06 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/09/06 10:00
PHST- 2002/09/06 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/09/06 10:00 [entrez]
AID - S138819810200269X [pii]
AID - 10.1016/s1388-1981(02)00269-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2002 Sep 5;1584(1):46-54. doi: 
      10.1016/s1388-1981(02)00269-x.