PMID- 12216616
OWN - NLM
STAT- MEDLINE
DCOM- 20030129
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 46
IP  - 1-2
DP  - 2002
TI  - Analysis of microbial community that performs enhanced biological phosphorus 
      removal in activated sludge fed with acetate.
PG  - 145-53
AB  - Enhanced biological phosphorus removal (EBPR) activated sludge was operated in a 
      laboratory-scale sequencing batch reactor (SBR) fed with acetate as the sole 
      carbon source. The microbial community of the sludge was analyzed using the 
      polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) 
      method for about 2 months of start-up period. As a result, the number of major 
      bands decreased during the enrichment, indicating that the microbial community 
      structure was getting simpler. Since the phosphate removal activity was 
      maintained at a high level, the bacteria which still remained at the end can be 
      considered as the important bacteria playing key roles in the present EBPR 
      sludge, maybe polyphosphate accumulating organisms (PAOs). The dominant band in 
      the last sample on the DGGE gel was excised and the DNA recovered from it was 
      sequenced. The sequence was closely related to one of the putative PAOs group 
      which Crocetti et al. (2000) and Hesselmann et al. (1999) have proposed. This 
      PAOs group is closely related to the Rhodocyclus group (b-Proteobacteria). The 
      fluorescence in situ hybridization (FISH) method with the probe specific for this 
      PAOs group and the DAPI staining at a phosphate-probing concentration indirectly 
      showed that these Rhodocyclus related bacteria really accumulated polyphosphate.
FAU - Onuki, M
AU  - Onuki M
AD  - Department of Urban and Environmental Engineering, University of Tokyo, Japan.
FAU - Satoh, H
AU  - Satoh H
FAU - Mino, T
AU  - Mino T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Polyphosphates)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Acetates/*chemistry
MH  - *Bioreactors
MH  - DNA, Bacterial/*analysis
MH  - Electrophoresis
MH  - Phosphorus/*metabolism
MH  - Polymerase Chain Reaction
MH  - Polyphosphates/*metabolism
MH  - Population Dynamics
MH  - Proteobacteria/*genetics/isolation & purification/physiology
MH  - Sequence Analysis, DNA
MH  - *Waste Disposal, Fluid
EDAT- 2002/09/10 10:00
MHDA- 2003/01/30 04:00
CRDT- 2002/09/10 10:00
PHST- 2002/09/10 10:00 [pubmed]
PHST- 2003/01/30 04:00 [medline]
PHST- 2002/09/10 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2002;46(1-2):145-53.