PMID- 12216618
OWN - NLM
STAT- MEDLINE
DCOM- 20030129
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 46
IP  - 1-2
DP  - 2002
TI  - Population dynamics in wastewater treatment plants with enhanced biological 
      phosphorus removal operated with and without nitrogen removal.
PG  - 163-70
AB  - The population dynamics of activated sludge in a pilot plant with two activated 
      sludge systems, both designed for enhanced biological phosphorus removal (EBPR), 
      but one of them with (BNP) and the other without (BP) nitrogen removal, was 
      monitored during a period of 2.5 years. The influent water to the pilot plant was 
      periodically manipulated by external addition of phosphorus (P), acetate and 
      glucose, respectively. The population dynamics and the in situ physiology were 
      monitored by quantitative fluorescence in situ hybridization (FISH) and 
      microautoradiography. Significant P removal was observed in both systems 
      throughout the whole period, with significant increases of the P removal when 
      substrates were dosed. The activated sludge in both systems contained large 
      amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods 
      during the whole period. A large part of the clusters belonged to the beta 
      Proteobacteria, whereas the rest of the clusters belonged either to the 
      Actinobacteria or to the alpha Proteobacteria. The relative abundance of 
      Rhodocyclus-related bacteria in the activated sludge varied significantly in both 
      systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). 
      However, no statistically significant correlation of the Rhodocyclus-related nor 
      any of the other investigated bacterial groups to the P content of the activated 
      sludge (correlation for all groups investigated was always < 0.5) was observed. A 
      significant 33Pi uptake was observed by the beta Proteobacteria (part of them 
      Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. 
      However, not all of the Rhodocyclus-related bacteria showed 33Pi uptake. The P 
      removal in the investigated plants is thus believed to be mediated by a mixed 
      population consisting of a part of the Rhodocyclus-related bacteria, the 
      Actinobacteria and other, yet unidentified bacteria.
FAU - Lee, N
AU  - Lee N
AD  - Lehrstuhl fur Mikrobiologie, TU Munchen, Freising, Germany. 
      www.microbial-ecology.de
FAU - Jansen, J la Cour
AU  - Jansen Jl
FAU - Aspegren, H
AU  - Aspegren H
FAU - Henze, M
AU  - Henze M
FAU - Nielsen, P H
AU  - Nielsen PH
FAU - Wagner, M
AU  - Wagner M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 27YLU75U4W (Phosphorus)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Autoradiography
MH  - *Bioreactors
MH  - Gram-Negative Bacteria
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen/metabolism
MH  - Phosphorus/*metabolism
MH  - Population Dynamics
MH  - *Waste Disposal, Fluid
EDAT- 2002/09/10 10:00
MHDA- 2003/01/30 04:00
CRDT- 2002/09/10 10:00
PHST- 2002/09/10 10:00 [pubmed]
PHST- 2003/01/30 04:00 [medline]
PHST- 2002/09/10 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2002;46(1-2):163-70.