PMID- 12216636
OWN - NLM
STAT- MEDLINE
DCOM- 20030129
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 46
IP  - 1-2
DP  - 2002
TI  - Molecular analysis of ammonia-oxidizing bacterial populations in aerated-anoxic 
      orbal processes.
PG  - 273-80
AB  - Aerated-anoxic processes operate under the principle that small additions of 
      oxygen to an anoxic reactor induce simultaneous nitrification and 
      denitrification. In these systems, ammonia oxidation in the anoxic zone can 
      easily account for 30-50% of the total nitrification in the reactor, even though 
      the dissolve oxygen concentration is usually below detection limit. To 
      investigate whether the nitrification efficiency in aerated-anoxic processes was 
      due to the presence of specialized ammonia-oxidizing bacteria (AOB), an analysis 
      of the AOB population in an aerated-anoxic Orbal process and a conventional 
      nitrogen removal process was carried out using phylogenetic analyses based on the 
      ammonia monooxygenase A (amoA) gene. Terminal restriction fragment length 
      polymorphism (TRFLP) analyses revealed that Nitrosospira-like organisms were one 
      of the major contributors to ammonia oxidation in a full-scale aerated-anoxic 
      Orbal reactor. However, the relative populations of Nitrosospira-like and 
      Nitrosomonas-like AOB were not constant and appeared to have seasonal 
      variability. Cloning and sequence comparison of amoA gene fragments demonstrated 
      that most of the AOB in the aerated-anoxic Orbal process belonged to the 
      Nitrosospira sp. and Nitrosomonas oligotropha lineages. The abundance of 
      Nitrosospira-like organisms in aerated-anoxic reactors is significant, since this 
      group of AOB has not been usually associated with nitrification in wastewater 
      treatment plants.
FAU - Park, H-D
AU  - Park HD
AD  - Department of Civil & Environmental Engineering, University of Wisconsin, Madison 
      53706, USA.
FAU - Regan, J M
AU  - Regan JM
FAU - Noguera, D R
AU  - Noguera DR
LA  - eng
GR  - 5 T32 GM08349/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/chemistry/*metabolism
MH  - *Bioreactors
MH  - DNA, Bacterial/analysis/*genetics
MH  - Nitrosomonas/*physiology
MH  - Oxidation-Reduction
MH  - Oxidoreductases/*genetics
MH  - Oxygen
MH  - Sequence Analysis, DNA
MH  - Waste Disposal, Fluid
EDAT- 2002/09/10 10:00
MHDA- 2003/01/30 04:00
CRDT- 2002/09/10 10:00
PHST- 2002/09/10 10:00 [pubmed]
PHST- 2003/01/30 04:00 [medline]
PHST- 2002/09/10 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2002;46(1-2):273-80.