PMID- 12218213
OWN - NLM
STAT- MEDLINE
DCOM- 20030213
LR  - 20051117
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 15
IP  - 9
DP  - 2002 Sep
TI  - Characteristic pattern of genetic aberrations in ovarian granulosa cell tumors.
PG  - 951-7
AB  - The cytogenetic abnormalities of granulosa cell tumors (GCT) of the ovary are 
      only partially known. Up to now, mainly numerical chromosomal aberrations have 
      been described. Therefore we performed a comprehensive study on paraffin-embedded 
      material of 20 GCT (17 adult, 3 juvenile; patient age between 16 and 78 y) 
      combining comparative genomic hybridization (CGH); fluorescence in situ 
      hybridization (FISH) using DNA-specific probes for chromosome 12, 17, 22, and X; 
      DNA cytometry; and immunohistochemistry (inhibin, p53, Ki67). By DNA cytometry, 
      16 of 20 tumors (80%) were diploid. However, 6 of 16 diploid tumors (37%) showed 
      aberrations by FISH. FISH revealed monosomy 22 in 8/18 cases (40%); trisomy 12 in 
      5/20 (25%); monosomy X in 2/20 (10%); and loss of chromosome 17 in one case (5%). 
      The main findings by CGH were gains of chromosomes 12 (6 cases, 33%) and 14 (6 
      cases, 33%) and losses of chromosomes 22 (7 cases, 35%) and X (1 case, 5%), 
      mostly comprising whole chromosomes or chromosome arms. Inhibin and p53 were 
      expressed in 100% and 95% of the tumors, respectively. The Ki67 index ranged from 
      0% to 61%. Neither immunohistochemistry, nor DNA cytometry and molecular genetic 
      analysis, provided statistically significant prognostic information. In summary, 
      our study reveals a distinctive pattern of cytogenetic alterations in GCT. Our 
      observations confirm earlier reports that trisomy 12 and 14 are frequent 
      aberrations; however, monosomy 22 seemingly is even more prevalent.
FAU - Mayr, D
AU  - Mayr D
AD  - Pathological Institute, University of Munich, Munich, Germany. 
      doris.mayr@lrz.uni-muenchen.de
FAU - Kaltz-Wittmer, C
AU  - Kaltz-Wittmer C
FAU - Arbogast, S
AU  - Arbogast S
FAU - Amann, G
AU  - Amann G
FAU - Aust, D E
AU  - Aust DE
FAU - Diebold, J
AU  - Diebold J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 57285-09-3 (Inhibins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Granulosa Cell Tumor/*genetics/metabolism/mortality
MH  - Humans
MH  - Image Cytometry
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Inhibins/biosynthesis
MH  - Ki-67 Antigen/biosynthesis
MH  - Middle Aged
MH  - Ovarian Neoplasms/*genetics/metabolism/mortality
MH  - Tumor Suppressor Protein p53/biosynthesis
EDAT- 2002/09/10 10:00
MHDA- 2003/02/14 04:00
CRDT- 2002/09/10 10:00
PHST- 2002/09/10 10:00 [pubmed]
PHST- 2003/02/14 04:00 [medline]
PHST- 2002/09/10 10:00 [entrez]
AID - 10.1097/01.MP.0000024290.55261.14 [doi]
PST - ppublish
SO  - Mod Pathol. 2002 Sep;15(9):951-7. doi: 10.1097/01.MP.0000024290.55261.14.