PMID- 12221059
OWN - NLM
STAT- MEDLINE
DCOM- 20020923
LR  - 20190623
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 106
IP  - 11
DP  - 2002 Sep 10
TI  - Detection of experimental autoimmune myocarditis in rats by 111In monoclonal 
      antibody specific for tenascin-C.
PG  - 1397-402
AB  - BACKGROUND: Although the identification of inflammatory infiltrates in 
      endomyocardial biopsy specimens is necessary for the definite diagnosis of 
      myocarditis, the biopsy test is invasive and is not sensitive. Therefore, a new 
      diagnostic technique for the early and noninvasive evaluation of myocarditis has 
      been awaited. Expression of tenascin-C (TNC), one of the oligometric 
      extracellular glycoproteins, is induced in various pathological states, including 
      inflammation, suggesting that TNC can be a molecular marker of myocarditis. 
      METHODS AND RESULTS: An 111In anti-TNC monoclonal antibody Fab' fragment was 
      injected intravenously into rats with experimental autoimmune myocarditis (EAM), 
      and the biodistribution of this radiotracer was measured. Rapid clearance of 
      radioactivity from the blood was observed in both EAM and control rats (<1% at 6 
      hours after injection). Myocardial uptake of the tracer was much higher in EAM 
      rats than in control rats (7.54-, 4.39-, and 3.51-fold at 6, 24, and 48 hours 
      after injection, respectively). By autoradiography, high radioactivities were 
      clearly observed in the regions indicative of inflammation in EAM rats. 
      Single-photon emission CT imaging demonstrated the focal myocardial uptake of 
      111In anti-TNC Fab' in vivo. CONCLUSIONS: Radiolabeled anti-TNC Fab' may be 
      useful for the noninvasive diagnosis of myocarditis.
FAU - Sato, Mikio
AU  - Sato M
AD  - Department of Cardiovascular Science and Medicine, Graduate School of Medicine, 
      Chiba University, Chiba-shi, Chiba, Japan.
FAU - Toyozaki, Tetsuya
AU  - Toyozaki T
FAU - Odaka, Kenichi
AU  - Odaka K
FAU - Uehara, Tomoya
AU  - Uehara T
FAU - Arano, Yasushi
AU  - Arano Y
FAU - Hasegawa, Hiroshi
AU  - Hasegawa H
FAU - Yoshida, Katsuya
AU  - Yoshida K
FAU - Imanaka-Yoshida, Kyoko
AU  - Imanaka-Yoshida K
FAU - Yoshida, Toshimichi
AU  - Yoshida T
FAU - Hiroe, Michiaki
AU  - Hiroe M
FAU - Tadokoro, Hiroyuki
AU  - Tadokoro H
FAU - Irie, Toshiaki
AU  - Irie T
FAU - Tanada, Shuji
AU  - Tanada S
FAU - Komuro, Issei
AU  - Komuro I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Indium Radioisotopes)
RN  - 0 (Tenascin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology/*pharmacokinetics
MH  - Autoimmune Diseases/diagnostic imaging/*metabolism/pathology
MH  - Autoradiography
MH  - Biomarkers/analysis
MH  - Female
MH  - Immunoglobulin Fab Fragments/metabolism
MH  - Indium Radioisotopes
MH  - Myocarditis/diagnostic imaging/*immunology/*metabolism/pathology
MH  - Myocardium/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Tenascin/*immunology/metabolism
MH  - Tomography, Emission-Computed, Single-Photon
EDAT- 2002/09/11 10:00
MHDA- 2002/09/24 06:00
CRDT- 2002/09/11 10:00
PHST- 2002/09/11 10:00 [pubmed]
PHST- 2002/09/24 06:00 [medline]
PHST- 2002/09/11 10:00 [entrez]
AID - 10.1161/01.cir.0000027823.07104.86 [doi]
PST - ppublish
SO  - Circulation. 2002 Sep 10;106(11):1397-402. doi: 
      10.1161/01.cir.0000027823.07104.86.