PMID- 12223762
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 114
IP  - 3
DP  - 1997 Jul
TI  - Expression of Pokeweed Antiviral Protein in Transgenic Plants Induces Virus 
      Resistance in Grafted Wild-Type Plants Independently of Salicylic Acid 
      Accumulation and Pathogenesis-Related Protein Synthesis.
PG  - 1113-1121
AB  - Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca 
      americana, inhibits translation by catalytically removing a specific adenine 
      residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. 
      Transgenic tobacco (Nicotiana tabacum) plants expressing PAP or a variant (PAP-v) 
      were shown to be resistant to a broad spectrum of plant viruses. Expression of 
      PAP-v in transgenic plants induces synthesis of pathogenesis-related proteins and 
      a very weak (<2-fold) increase in salicylic acid levels. Using reciprocal 
      grafting experiments, we demonstrate here that transgenic tobacco rootstocks 
      expressing PAP-v induce resistance to tobacco mosaic virus infection in both N. 
      tabacum NN and nn scions. Increased resistance to potato virus X was also 
      observed in N. tabacum nn scions grafted on transgenic rootstocks. PAP expression 
      was not detected in the wild-type scions or rootstocks that showed virus 
      resistance, nor was there any increase in salicylic acid levels or 
      pathogenesis-related protein synthesis. Grafting experiments with transgenic 
      plants expressing an inactive PAP mutant demonstrated that an intact active site 
      of PAP is necessary for induction of virus resistance in wild-type scions. These 
      results indicate that enzymatic activity of PAP is responsible for generating a 
      signal that renders wild-type scions resistant to virus infection in the absence 
      of increased salicylic acid levels and pathogenesis-related protein synthesis.
FAU - Smirnov, S
AU  - Smirnov S
AD  - Center for Agricultural Molecular Biology and Department of Plant Pathology, 
      Rutgers University, P.O. Box 231, New Brunswick, New Jersey 08903-0231.
FAU - Shulaev, V
AU  - Shulaev V
FAU - Tumer, N E
AU  - Tumer NE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
PMC - PMC158401
EDAT- 1997/07/01 00:00
MHDA- 2002/09/12 10:00
PMCR- 1998/07/01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 2002/09/12 10:00 [medline]
PHST- 1997/07/01 00:00 [entrez]
PHST- 1998/07/01 00:00 [pmc-release]
AID - 114/3/1113 [pii]
AID - 10.1104/pp.114.3.1113 [doi]
PST - ppublish
SO  - Plant Physiol. 1997 Jul;114(3):1113-1121. doi: 10.1104/pp.114.3.1113.