PMID- 12225812
OWN - NLM
STAT- MEDLINE
DCOM- 20020926
LR  - 20191106
IS  - 0021-5155 (Print)
IS  - 0021-5155 (Linking)
VI  - 46
IP  - 4
DP  - 2002 Jul-Aug
TI  - High level of Fc epsilon receptor I-bindable immunoglobulin E in the tear fluid 
      and increased immunoglobulin E-saturated cells in the giant papillae of vernal 
      keratoconjunctivitis patients.
PG  - 357-63
AB  - PURPOSE: To investigate the concentrations of Fc epsilon receptor I (Fc epsilon 
      RI)-bindable immunoglobulin E (IgE) in the tear fluid and the proportion of 
      IgE-saturated cells among Fc epsilon RI-positive cells in giant papillae from 
      vernal keratoconjunctivitis (VKC) patients. METHODS: The tear fluids and giant 
      papillae were obtained from 8 VKC patients with their informed consent. To detect 
      the quantitative difference between Fc epsilon RI-bindable IgE and total IgE in 
      the tear fluid, we used a new enzyme-linked immunosorbent assay system we have 
      developed. Next, to estimate the proportion of IgE-saturated cells among Fc 
      epsilon RI-positive cells, we used two distinct monoclonal antibodies for Fc 
      epsilon RI for the immunohistochemistry. One antibody recognizes all Fc epsilon 
      RI regardless of whether it is with or without receptor-bound IgE. The other does 
      not recognize IgE-bound Fc epsilon RI. RESULTS: The quantitative difference 
      between Fc epsilon RI-bindable IgE and total IgE were detected in the tear fluid 
      of VKC patients. Fc epsilon RI-positive cells were significantly increased in the 
      giant papillae of VKC compared with normal conjunctivae. The proportion of 
      IgE-saturated cells among Fc epsilon RI-positive cells in giant papillae was 
      higher than that in normal conjunctivae. CONCLUSION: These results suggest that F 
      epsilon RI-bindable IgE may be a critical factor to estimate the severity of VKC.
FAU - Ebihara, Nobuyuki
AU  - Ebihara N
AD  - Departments of Ophthalmology, Juntendo University School of Medicine, Tokyo, 
      Japan.
FAU - Okumura, Ko
AU  - Okumura K
FAU - Nakayasu, Kiyoo
AU  - Nakayasu K
FAU - Kanai, Atsushi
AU  - Kanai A
FAU - Ra, Chisei
AU  - Ra C
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Cell Count
MH  - Conjunctivitis, Allergic/*immunology/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin E/*immunology
MH  - Receptors, IgE/*immunology
MH  - Tears/*immunology
EDAT- 2002/09/13 10:00
MHDA- 2002/09/27 06:00
CRDT- 2002/09/13 10:00
PHST- 2002/09/13 10:00 [pubmed]
PHST- 2002/09/27 06:00 [medline]
PHST- 2002/09/13 10:00 [entrez]
AID - S0021515502005075 [pii]
AID - 10.1016/s0021-5155(02)00507-5 [doi]
PST - ppublish
SO  - Jpn J Ophthalmol. 2002 Jul-Aug;46(4):357-63. doi: 10.1016/s0021-5155(02)00507-5.