PMID- 12227276
OWN - NLM
STAT- MEDLINE
DCOM- 20021219
LR  - 20191025
IS  - 0941-293X (Print)
IS  - 0941-293X (Linking)
VI  - 99
IP  - 8
DP  - 2002 Aug
TI  - [HLA class I antigen expression correlates with histological cell type in uveal 
      melanoma].
PG  - 625-8
AB  - PURPOSE: The prognosis of uveal melanoma is correlated with its histologic cell 
      type. The epithelioid cell type is associated with a higher metastatic rate than 
      the spindle cell type. The Human Leucocyte Antigen Class I (HLA-I) expression of 
      the melanoma also correlates with the prognosis. In this study, we analyzed HLA-I 
      antigen expression of uveal melanomas to determine whether a relationship exist 
      between antigenic expression and melanoma cell type. METHODS: Formalin-fixed, 
      paraffin-embedded spindle cell type (n = 11) and epithelioid cell type (n = 11) 
      uveal melanomas were immunostained with the HC10 antibody (1:80) for HLA-I 
      antigen expression with appropriate positive and negative controls. Sections were 
      assessed semiquantitatively according to the percentage of stained cells. 
      RESULTS: Among the spindle cell type melanomas, 2 out of 11 (18%) stained with 
      HC10 antibodies. The staining intensity was less than 25% of the cells in these 
      two melanomas. Among the epithelioid cell type melanomas, 9 out of 11 (82%) 
      stained with HC10. The staining intensity was more than 25% of the cells in 5 of 
      these 9 melanomas. CONCLUSIONS: It is unknown why spindle and epithelioid cell 
      type uveal melanomas have different prognoses. Human uveal melanoma cell lines 
      with low HLA-I expression are susceptible to NK cell-mediated lysis in vitro and 
      in murine studies. The prognostically more favorable spindle cell type melanoma 
      expresses less HLA-I than the epithelioid cell type melanoma. These results 
      stress the role of NK cells in the rejection of uveal melanoma.
FAU - Dithmar, S
AU  - Dithmar S
AD  - Universitats-Augenklinik Heidelberg, lm Neuenheimer Feld 400, 69120 Heidelberg.
FAU - Crowder, J
AU  - Crowder J
FAU - Jager, M J
AU  - Jager MJ
FAU - Vigniswaran, N
AU  - Vigniswaran N
FAU - Grossniklaus, H E
AU  - Grossniklaus HE
LA  - ger
GR  - P30 EY06360/EY/NEI NIH HHS/United States
GR  - R01 EY13165/EY/NEI NIH HHS/United States
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
TT  - HLA-I-Antigenexpression korreliert mit dem histologischen Zelltyp uvealer 
      Melanome.
PL  - Germany
TA  - Ophthalmologe
JT  - Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
JID - 9206148
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Melanoma/classification/*pathology
MH  - Prognosis
MH  - Uvea/pathology
MH  - Uveal Neoplasms/classification/*pathology
EDAT- 2002/09/14 10:00
MHDA- 2002/12/20 04:00
CRDT- 2002/09/14 10:00
PHST- 2002/09/14 10:00 [pubmed]
PHST- 2002/12/20 04:00 [medline]
PHST- 2002/09/14 10:00 [entrez]
AID - 10.1007/s00347-001-0596-3 [doi]
PST - ppublish
SO  - Ophthalmologe. 2002 Aug;99(8):625-8. doi: 10.1007/s00347-001-0596-3.