PMID- 12231372
OWN - NLM
STAT- MEDLINE
DCOM- 20021218
LR  - 20190922
IS  - 1053-2498 (Print)
IS  - 1053-2498 (Linking)
VI  - 21
IP  - 9
DP  - 2002 Sep
TI  - The use of (99m)technetium-labeled MCP-1 to assess graft coronary artery disease 
      in rat cardiac allografts.
PG  - 1009-15
AB  - BACKGROUND: Monocyte chemoattractant protein-1 (MCP-1) is associated with the 
      development of graft coronary artery disease (GCAD) following cardiac 
      transplantation. This study assessed whether technetium 99m ((99m)Tc)-labeled 
      MCP-1 binds its receptors in chronic cardiac transplants and thereby provides a 
      potential modality to assess GCAD. METHODS: Allogeneic (PVG-->ACI, n = 9) and 
      syngeneic (ACI-->ACI, n = 9) rat heterotopic heart transplants were performed. 
      Allograft recipients were treated with 7.5 mg/kg per day of Cyclosporin A for 10 
      days until tolerance was achieved. After 90 days, animals were injected 
      intravenously with (99m)Tc-MCP-1 and killed after 1 hour. Radioactivity of heart 
      tissues was measured and standardized to uptake in the overall blood pool. 
      Two-dimensional (99m)Tc-MCP-1 uptake (autoradiographs) was imaged by exposing 
      50-microm sections on a phosphoimager overnight. ED-1 staining of 
      monocyte/macrophages was performed on serial sections. Additional sections were 
      stained with elastin von Gieson and hematoxylin. Hearts were scored for luminal 
      narrowing and intima/media ratio (I/M) with computerized image analysis. RESULTS: 
      Allografts exhibited significantly more luminal narrowing (22.5 +/- 10.7% vs 2.6 
      +/- 4.6, p = 0.0005) and higher I/M (0.173 +/- 0.151 vs 0.015 +/- 0.029, p = 
      0.0088) than isografts. The ratio of (99m)Tc-MCP-1 uptake in allografts (1.04 +/- 
      0.4) was greater than that of isograft controls (0.72 +/- 0.11, p = 0.03). Pixel 
      counts of autoradiographs and ED-1-stained sections demonstrated a modest 
      correlation between the two (R(2) = 0.50). No significant differences were seen 
      in acute rejection scores. CONCLUSION: (99m)Tc-MCP-1 uptake was higher in 
      allografts vs isografts and was consistent with a greater degree of GCAD. These 
      data demonstrating increased radiopharmaceutical uptake in hearts with GCAD 
      provide a foundation for the development of a potentially non-invasive imaging 
      assay of this disease process in heart transplantation.
FAU - Kown, Murray H
AU  - Kown MH
AD  - Department of Cardiothoracic Surgery, Stanford University School of Medicine, 
      California 94305, USA.
FAU - Jahncke, Christina L
AU  - Jahncke CL
FAU - Lijkwan, Maarten A
AU  - Lijkwan MA
FAU - Koransky, Mark L
AU  - Koransky ML
FAU - Mari, Carina
AU  - Mari C
FAU - Berry, Gerald J
AU  - Berry GJ
FAU - Blankenberg, Francis G
AU  - Blankenberg FG
FAU - Strauss, H William
AU  - Strauss HW
FAU - Robbins, Robert C
AU  - Robbins RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Heart Lung Transplant
JT  - The Journal of heart and lung transplantation : the official publication of the 
      International Society for Heart Transplantation
JID - 9102703
RN  - 0 (Chemokine CCL2)
RN  - 0 (Radiopharmaceuticals)
RN  - 7440-26-8 (Technetium)
SB  - IM
MH  - Animals
MH  - *Chemokine CCL2/metabolism
MH  - Coronary Artery Disease/*immunology/metabolism/pathology
MH  - Graft Occlusion, Vascular/*immunology/metabolism/pathology
MH  - Heart Transplantation/*adverse effects
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Male
MH  - *Radiopharmaceuticals/metabolism
MH  - Rats
MH  - *Technetium
EDAT- 2002/09/17 10:00
MHDA- 2002/12/19 04:00
CRDT- 2002/09/17 10:00
PHST- 2002/09/17 10:00 [pubmed]
PHST- 2002/12/19 04:00 [medline]
PHST- 2002/09/17 10:00 [entrez]
AID - S1053249802004217 [pii]
AID - 10.1016/s1053-2498(02)00421-7 [doi]
PST - ppublish
SO  - J Heart Lung Transplant. 2002 Sep;21(9):1009-15. doi: 
      10.1016/s1053-2498(02)00421-7.