PMID- 12231435
OWN - NLM
STAT- MEDLINE
DCOM- 20021217
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 330
IP  - 2
DP  - 2002 Sep 20
TI  - Low levels of brain-derived neurotrophic factor and tyrosine kinase receptor B 
      are related to loss of dentate granule cells after prolonged low-protein feeding 
      in the rat.
PG  - 155-8
AB  - Protein deprivation leads to neuronal and synaptic loss in the hippocampal 
      formation, and to behavioral changes. We suggested that these effects could 
      result from alterations in the levels of brain-derived neurotrophic factor (BDNF) 
      and tyrosine kinase receptor B (TrkB). To investigate this issue, adult rats were 
      submitted to protein deprivation for 6 months and compared with controls. The 
      number of neurons of the dentate gyrus granular layer containing BDNF and TrkB 
      was estimated from immunostained sections and the mRNA levels of BDNF and TrkB 
      evaluated using in situ hybridization. After treatment, there was a loss of BDNF- 
      and TrkB-immunoreactive cells and a reduction of the mRNA levels. Thus, it is 
      likely that the decreased neurotrophic activity in the dentate gyrus of 
      malnourished animals underpins neuronal degeneration and the ensuing behavioral 
      alterations.
FAU - Mesquita, Rui M
AU  - Mesquita RM
AD  - Department of Anatomy, Porto Medical School, Al. Prof. Hernani Monteiro, 4200-319 
      Porto, Portugal.
FAU - Pereira, Pedro A
AU  - Pereira PA
FAU - Andrade, Jose P
AU  - Andrade JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Dietary Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Dentate Gyrus/cytology/drug effects/*metabolism
MH  - Diet, Protein-Restricted
MH  - Dietary Proteins/*administration & dosage
MH  - Gene Expression
MH  - Hippocampus/cytology/drug effects/metabolism
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/genetics/*metabolism
EDAT- 2002/09/17 10:00
MHDA- 2002/12/18 04:00
CRDT- 2002/09/17 10:00
PHST- 2002/09/17 10:00 [pubmed]
PHST- 2002/12/18 04:00 [medline]
PHST- 2002/09/17 10:00 [entrez]
AID - S0304394002007437 [pii]
AID - 10.1016/s0304-3940(02)00743-7 [doi]
PST - ppublish
SO  - Neurosci Lett. 2002 Sep 20;330(2):155-8. doi: 10.1016/s0304-3940(02)00743-7.