PMID- 12231440
OWN - NLM
STAT- MEDLINE
DCOM- 20021217
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 330
IP  - 2
DP  - 2002 Sep 20
TI  - Brain derived neurotrophic factor does not act on adult human cerebral 
      endothelial cells.
PG  - 175-8
AB  - Brain derived neurotrophic factor (BDNF), known to promote survival of neurons, 
      has recently been involved in the regulation of endothelial cells and was shown 
      to possess immunoregulatory properties. We therefore asked whether BDNF has 
      immunoregulating effects in human cerebral endothelial cells (HCEC). Adult HCEC 
      expressed vascular cell adhesion molecule-1 and HLA class II following 
      stimulation with tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma, 
      respectively, but not after BDNF treatment. Addition of BDNF did not modulate the 
      effects of TNF-alpha and IFN-gamma on HCEC, including TNF-alpha induced 
      interleukin-8. Fluorescent activated cell sorting, RT-PCR, and 
      immunohistochemistry indicated that HCEC do not express the trkB or p75 receptors 
      involved in BDNF signaling. These results demonstrate that adult HCEC are not a 
      likely target for BDNF. Novel factors regulating HCEC remain to be identified.
FAU - Ruprecht, Klemens
AU  - Ruprecht K
AD  - Clinical Research Unit for Multiple Sclerosis and Neuroimmunology, Department of 
      Neurology, Julius Maximilians Universitat, 97080 Wurzburg, Germany. 
      klemens.ruprecht@mail.uni-wuerzburg.de
FAU - Stadelmann, Christine
AU  - Stadelmann C
FAU - Hummel, Vera
AU  - Hummel V
FAU - Klein, Oliver
AU  - Klein O
FAU - Bruck, Wolfgang
AU  - Bruck W
FAU - Rieckmann, Peter
AU  - Rieckmann P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (HLA Antigens)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Endothelium, Vascular/cytology/*drug effects/metabolism
MH  - Flow Cytometry
MH  - Gene Expression
MH  - HLA Antigens/biosynthesis/drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-18/pharmacology
MH  - Interleukin-8/biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptor, Nerve Growth Factor
MH  - Receptor, trkB/biosynthesis/drug effects/genetics
MH  - Receptors, Nerve Growth Factor/biosynthesis/drug effects/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - Vascular Cell Adhesion Molecule-1/biosynthesis/drug effects
EDAT- 2002/09/17 10:00
MHDA- 2002/12/18 04:00
CRDT- 2002/09/17 10:00
PHST- 2002/09/17 10:00 [pubmed]
PHST- 2002/12/18 04:00 [medline]
PHST- 2002/09/17 10:00 [entrez]
AID - S0304394002007784 [pii]
AID - 10.1016/s0304-3940(02)00778-4 [doi]
PST - ppublish
SO  - Neurosci Lett. 2002 Sep 20;330(2):175-8. doi: 10.1016/s0304-3940(02)00778-4.