PMID- 12234994
OWN - NLM
STAT- MEDLINE
DCOM- 20021008
LR  - 20201215
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 62
IP  - 18
DP  - 2002 Sep 15
TI  - Adenovirus infection enhances dendritic cell immunostimulatory properties and 
      induces natural killer and T-cell-mediated tumor protection.
PG  - 5260-6
AB  - Dendritic cells (DCs) are potent antigen-presenting cells with the potential for 
      cancer immunotherapy. Adenoviral-mediated gene transfer is an attractive means to 
      manipulate the immunostimulatory properties of DCs for therapeutic advantage. 
      Because adenovirus induces DC maturation, we postulated that it would 
      significantly alter their immune functions. Infected DCs markedly increased 
      allogeneic and antigen-specific T-cell proliferation, and augmented natural 
      killer cell lytic activity and IFN-gamma production. The enhanced effector cell 
      stimulation by infected DCs was dependent on their secretion of interleukin 12. 
      Immunization with infected DCs pulsed with tumor antigen protected against flank 
      tumors in 78% of mice and induced a memory response. Antitumor immunity was 
      dependent on both T cells and natural killer cells. Antigen-pulsed, mock-infected 
      DCs were nonprotective. The findings that adenoviral vectors alone critically 
      alter DC immune functions and antitumor properties have important implications 
      for the design and interpretation of immunotherapy regimens using vector-based 
      gene transfer to modulate immunity.
FAU - Miller, George
AU  - Miller G
AD  - Hepatobiliary Service, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 
      New York, NY 10021, USA.
FAU - Lahrs, Svenja
AU  - Lahrs S
FAU - Pillarisetty, Venu G
AU  - Pillarisetty VG
FAU - Shah, Alaap B
AU  - Shah AB
FAU - DeMatteo, Ronald P
AU  - DeMatteo RP
LA  - eng
GR  - CA 94503/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Antigens, Neoplasm)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adenoviridae/*immunology
MH  - Animals
MH  - Antigens, Neoplasm/immunology/metabolism
MH  - Chemotaxis/immunology
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology/virology
MH  - Immunotherapy, Adoptive/methods
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-12/biosynthesis/immunology/metabolism
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Lymphoma/immunology/therapy
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes/*immunology
EDAT- 2002/09/18 10:00
MHDA- 2002/10/09 04:00
CRDT- 2002/09/18 10:00
PHST- 2002/09/18 10:00 [pubmed]
PHST- 2002/10/09 04:00 [medline]
PHST- 2002/09/18 10:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2002 Sep 15;62(18):5260-6.