PMID- 12235273
OWN - NLM
STAT- MEDLINE
DCOM- 20021021
LR  - 20220227
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 303
IP  - 1
DP  - 2002 Oct
TI  - Role of the nitric oxide pathway in kappa-opioid-induced hypothermia in rats.
PG  - 375-8
AB  - The effect of central and peripheral administration of a nitric oxide synthase 
      inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on the hypothermia induced 
      by the selective kappa-opioid receptor agonist 
      trans-(+/-)3,4-dichloro-N-methyl-N-(2-[1-pyrrolidinyl]-cyclohexyl)-benzeneacetamide 
      methane sulfate (U50,488H) was studied in male Sprague-Dawley rats. In the first 
      series of experiments, we examined the effect of subcutaneous (s.c.) 
      administration of L-NAME on the hypothermia induced by s.c. injection of 
      U50,488H. L-NAME, at a dose of 50 mg/kg s.c., had no influence on body 
      temperature (Tb). Coadministration of L-NAME (50 mg/kg, s.c.) with U50,488H (10 
      mg/kg, s.c.) blocked the hypothermia induced by U50,488H. In the second series of 
      experiments, we investigated the effect of intracerebroventricular (i.c.v.) 
      administration of L-NAME on the hypothermia induced by s.c. injection of 
      U50,488H. L-NAME itself, given i.c.v. at a dose of 1 mg/rat, did not evoke any 
      change in Tb. Administration of L-NAME (1 mg/rat, i.c.v.) caused a significant 
      suppression of U50,488H hypothermia. The results indicate that either central or 
      peripheral nitric oxide synthesis is required for the production of hypothermia 
      induced by U50,488H.
FAU - Benamar, Khalid
AU  - Benamar K
AD  - Center for Substance Abuse Research and Department of Pharmacology, Temple 
      University School of Medicine, Philadelphia, Pennsylvania 19140, USA. 
      kbenamar@hotmail.com
FAU - Geller, Ellen B
AU  - Geller EB
FAU - Adler, Martin W
AU  - Adler MW
LA  - eng
GR  - DA00376/DA/NIDA NIH HHS/United States
GR  - DA13429/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Analgesics, Non-Narcotic)
RN  - 0 (Receptors, Opioid, kappa)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 67198-13-4 
      (3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, 
      (trans)-Isomer)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, 
      (trans)-Isomer/administration & dosage/*pharmacology
MH  - Analgesics, Non-Narcotic/administration & dosage/pharmacology
MH  - Animals
MH  - Body Temperature/drug effects
MH  - Cerebral Ventricles/drug effects/physiopathology
MH  - Hypothermia/*physiopathology
MH  - Injections, Intraventricular
MH  - Male
MH  - NG-Nitroarginine Methyl Ester/administration & dosage/*pharmacology
MH  - Nitric Oxide/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Opioid, kappa/*physiology
EDAT- 2002/09/18 10:00
MHDA- 2002/10/22 04:00
CRDT- 2002/09/18 10:00
PHST- 2002/09/18 10:00 [pubmed]
PHST- 2002/10/22 04:00 [medline]
PHST- 2002/09/18 10:00 [entrez]
AID - 10.1124/jpet.102.036269 [doi]
PST - ppublish
SO  - J Pharmacol Exp Ther. 2002 Oct;303(1):375-8. doi: 10.1124/jpet.102.036269.