PMID- 12235897
OWN - NLM
STAT- MEDLINE
DCOM- 20021022
LR  - 20061115
IS  - 0034-9887 (Print)
IS  - 0034-9887 (Linking)
VI  - 130
IP  - 7
DP  - 2002 Jul
TI  - [Pilot study of PML/RAR alpha fusion by fluorescence in situ hybridization 
      (FISH)method in acute promyelocyte leukemia].
PG  - 737-44
AB  - BACKGROUND: Acute promyelocytic leukemia (APL) is characterized cytogenetically 
      by t(15;17) (q22;q21) and its molecular consequence, fusion of PML and RAR alpha 
      genes. The detection of this genetic marker confirms the diagnosis and allows 
      monitoring of the leukemic clone during treatment, which has prognostic value. 
      Cytogenetics fails in some cases due to the absence of metaphases in cultures or 
      their bad morphology. Southern blot and PCR methods require trained personnel and 
      adequate equipment. FISH method allows the identification of chromosomic 
      rearrangements in 24 to 48 h and is simple to set up in a cytogenetics 
      laboratory. AIM: To evaluate the FISH method to detect PML/RAR alpha fusion, 
      compared to cytogenetic analysis. PATIENTS AND METHODS: Fifteen bone marrow 
      specimens from APL patients with previous cytogenetic analysis were studied, 
      using a commercial probe to detect PML/RAR alpha fusion. RESULTS: We obtained a 
      normal cut-off value of 9.1%. Specificity and sensibility were 100%. Six positive 
      cytogenetic cases at diagnosis were FISH positive. Six negative cytogenetic 
      cases, one APL at diagnosis and five normal controls were FISH negative. One case 
      in remission, that was negative by cytogenetics, was positive near the cut-off 
      value by FISH. Two other cases in remission, not conclusive by cytogenetics, were 
      negative by FISH. CONCLUSIONS: FISH is a reliable, rapid and relatively low cost 
      method that can be used as an adjunct to conventional cytogenetics.
FAU - Legues, Maria Eugenia
AU  - Legues ME
AD  - Laboratorio de Hematologia, Departamento de Hematologia-Oncologia, Pontificia 
      Universidad Catolica de Chile, Lira 44, Santiago.
FAU - Franco, Giannina
AU  - Franco G
FAU - Bertin, Pablo
AU  - Bertin P
LA  - spa
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
TT  - Estudio piloto de la fusion PML/RAR alpha por el metodo de hibridacion in situ 
      con fluorescencia (FISH) en leucemia aguda promielocitica.
PL  - Chile
TA  - Rev Med Chil
JT  - Revista medica de Chile
JID - 0404312
RN  - 0 (Genetic Markers)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein)
SB  - IM
MH  - Cytogenetic Analysis
MH  - Genetic Markers
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/diagnosis/*genetics
MH  - Neoplasm Proteins/*analysis/genetics
MH  - Oncogene Proteins, Fusion/*analysis/genetics
MH  - Pilot Projects
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
EDAT- 2002/09/19 10:00
MHDA- 2002/10/31 04:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
PST - ppublish
SO  - Rev Med Chil. 2002 Jul;130(7):737-44.