PMID- 12237190
OWN - NLM
STAT- MEDLINE
DCOM- 20021206
LR  - 20190818
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 99
IP  - 1-2
DP  - 2002 Sep
TI  - Expression of neurotrophic factors in the dorsal root ganglion in a rat model of 
      lumbar disc herniation.
PG  - 121-32
AB  - A variety of molecules released by inflammatory reactions in the dorsal root and 
      dorsal root ganglion (DRG) may play important roles in the pathology of neuronal 
      abnormalities in lumbar disc herniation. In order to elucidate the 
      pathophysiological mechanisms of painful radiculopathy, secondary to lumbar disc 
      herniation, we evaluated pain-related behavior and the change of nerve growth 
      factor (NGF) and brain-derived neurotrophic factor (BDNF) expression in the DRG 
      and dorsal root using a rat model of lumbar disc herniation. In the nucleus 
      pulposus (NP) group, the left L4/5 nerve roots were exposed after 
      hemilaminectomies and autologous intervertebral discs, which were obtained from 
      coccygeal intervertebral discs, were implanted on each of the exposed nerve roots 
      without mechanical compression. Rats in the NP group, but not the sham-operated 
      rats, developed mechanical allodynia on the ipsilateral hind paw for 1 day after 
      surgery and showed a significant increase in the number of NGF-immunoreactive 
      (IR) cells in the nerve root and DRG. NGF-IR cells in the nerve root and DRG 
      included macrophages and Schwann cells, because these cells were labeled for NGF 
      and ED-1 or glial fibrillary acid protein by dual immunostaining. A significant 
      increase in the percentage of BDNF-IR neurons in the DRG was observed in the NP 
      group at 3 days after surgery and the increase in BDNF mRNA expression was 
      confirmed using in situ hybridization histochemistry and reverse 
      transcription-polymerase chain reaction. We also injected NGF into the 
      endoneurial space of the normal rat spinal nerve root and found that the NGF 
      injection produced dose-dependent mechanical allodynia on the ipsilateral hind 
      paw at 1 day after surgery and an increase in the percentage of BDNF-IR neurons 
      in the DRG at 3 days after surgery compared to the group receiving saline 
      injection. These findings suggest that in the lumbar disc herniation model, i.e. 
      neuritis of the nerve root, increased NGF produced by the inflammatory responses 
      in the dorsal root and DRG tissues may affect the production of BDNF in the DRG 
      and may play important roles in the modulation of the dorsal horn neurons. These 
      changes in neurotrophic factors in the primary afferents may be involved in the 
      pathophysiological mechanisms of neuropathic pain produced by lumbar disc 
      herniation.
CI  - Copyright 2002 International Association for the Study of Pain
FAU - Obata, Koichi
AU  - Obata K
AD  - Department of Anatomy and Neuroscience, Hyogo College of Medicine, 1-1 
      Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
FAU - Tsujino, Hiroaki
AU  - Tsujino H
FAU - Yamanaka, Hiroki
AU  - Yamanaka H
FAU - Yi, Dai
AU  - Yi D
FAU - Fukuoka, Tetsuo
AU  - Fukuoka T
FAU - Hashimoto, Norio
AU  - Hashimoto N
FAU - Yonenobu, Kazuo
AU  - Yonenobu K
FAU - Yoshikawa, Hideki
AU  - Yoshikawa H
FAU - Noguchi, Koichi
AU  - Noguchi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - Disease Models, Animal
MH  - Ganglia, Spinal/cytology/*physiology
MH  - Gene Expression/physiology
MH  - Glial Fibrillary Acidic Protein/analysis
MH  - Intervertebral Disc Displacement/*complications
MH  - Lumbar Vertebrae
MH  - Male
MH  - Nerve Growth Factor/analysis/*genetics/pharmacology
MH  - Neuralgia/*physiopathology
MH  - Neuritis/physiopathology
MH  - Neurons, Afferent/chemistry/physiology
MH  - Peripheral Nerves/drug effects
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/09/19 10:00
MHDA- 2002/12/07 04:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2002/12/07 04:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
AID - S0304395902000684 [pii]
AID - 10.1016/s0304-3959(02)00068-4 [doi]
PST - ppublish
SO  - Pain. 2002 Sep;99(1-2):121-32. doi: 10.1016/s0304-3959(02)00068-4.