PMID- 12237195
OWN - NLM
STAT- MEDLINE
DCOM- 20021206
LR  - 20190818
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 99
IP  - 1-2
DP  - 2002 Sep
TI  - Partial sciatic nerve ligation induces increase in the phosphorylation of 
      extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in 
      astrocytes in the lumbar spinal dorsal horn and the gracile nucleus.
PG  - 175-84
AB  - The activation of glial cells in the spinal dorsal horn and the gracile nucleus 
      by inflammation and nerve injury has been suggested to be involved in neuronal 
      plasticity and central sensitization, hence contributing to tactile allodynia. 
      The aim of this study was to determine the possible intracellular signal 
      transduction pathway associated with glial cells, which have been activated by 
      partial sciatic nerve ligation (PSNL), a well-characterized rat model of 
      neuropathic pain. At 3 weeks post-lesion, PSNL markedly increased glia fibrillary 
      acidic protein (GFAP) immunoreactive (IR) astrocytes in both the L4-5 spinal 
      dorsal horn and the gracile nucleus. Moreover, PSNL increased the phosphorylation 
      of mitogen activated protein (MAP) kinases, including the extracellular 
      signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK), but not p38, in 
      glia-like cells in these same areas. Both phosphorylated (p) ERK- and JNK-IR 
      cells were co-localized with GFAP, suggesting their expression in reactive 
      astrocytes. In summary, our data indicate that PSNL activates ERK/MAP and JNK/MAP 
      kinase pathways in astrocytes in the dorsal horn and the gracile nucleus, these 
      events possibly being involved in the pathogenesis of neuropathic pain.
CI  - Copyright 2002 International Association for the Study of Pain
FAU - Ma, Weiya
AU  - Ma W
AD  - Department of Psychiatry, Douglas Hospital Research Center, McGill University, 
      6875 Boulevard LaSalle, Verdun, Montreal, Quebec, Canada H4H 1R3.
FAU - Quirion, Remi
AU  - Quirion R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Astrocytes/*enzymology
MH  - Cell Count
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Immunohistochemistry
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Ligation
MH  - Male
MH  - Medulla Oblongata/cytology/*metabolism
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/physiology
MH  - Sciatica/*metabolism
MH  - Spinal Cord/cytology/*metabolism
MH  - Touch
EDAT- 2002/09/19 10:00
MHDA- 2002/12/07 04:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2002/12/07 04:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
AID - S0304395902000970 [pii]
AID - 10.1016/s0304-3959(02)00097-0 [doi]
PST - ppublish
SO  - Pain. 2002 Sep;99(1-2):175-84. doi: 10.1016/s0304-3959(02)00097-0.