PMID- 12237233
OWN - NLM
STAT- MEDLINE
DCOM- 20020924
LR  - 20191210
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 136
IP  - 2
DP  - 2002 Jul 15
TI  - Banded chromosomes versus fluorescence in situ hybridization in the diagnosis of 
      mantle cell lymphoma: a lesson from three cases.
PG  - 108-12
AB  - We present three cases with presumptive evidence of mantle cell lymphoma (MCL) 
      that were submitted for cytogenetic evaluation. Chromosome analysis showed a 
      normal karyotype in two cases, while the third case showed the composite 
      karyotype; 45,XY,t(1;22)(p13;q13),23,del(10)(q22),add(15)(q22),add(17)(p13). The 
      characteristic t(11;14)(q13;q32) for MCL was not observed by conventional 
      karyotyping in any of the cases. We furthermore evaluated the specimens by 
      fluorescence in situ hybridization (FISH) using the dual-color LSI IgH/CCND1 DNA 
      probe. Fusion signals, consistent with t(11;14)(q13;q32), were observed in 65% 
      and 85% of interphase cells in cases 1 and 2, respectively, while the metaphases 
      from both cases revealed a normal pattern. All abnormal metaphases as well as 57% 
      of interphase cells from case 3 displayed a fusion signal. In the abnormal 
      metaphase cells, the fused signal was located on the normally looking 14q32, 
      suggesting that the IgH/CCND1 fusion resulted from the insertion of the CCND1 
      gene into 14q32 adjacent to the IgH gene. Thus, FISH confirmed the diagnosis of 
      MCL by showing the IgH/CCND1 fusion. In addition, these findings indicate that 
      the sensitivity of FISH is superior to that of conventional cytogenetics in 
      detecting t(11;14)(q13;q32) associated with MCL.
FAU - Mohamed, Anwar N
AU  - Mohamed AN
AD  - Cytogenetics Laboratory, Department of Pathology, Wayne State University, 
      Detroit, MI, USA. anwar_mohamed@hotmail.com
FAU - Ali, Wassim
AU  - Ali W
FAU - Kopptich, Fred
AU  - Kopptich F
FAU - al Katib, Ayad
AU  - al Katib A
LA  - eng
PT  - Case Reports
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 11
MH  - Chromosomes, Human, Pair 14
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphoma, Mantle-Cell/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
EDAT- 2002/09/19 10:00
MHDA- 2002/09/25 06:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2002/09/25 06:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
AID - S0165460802005241 [pii]
AID - 10.1016/s0165-4608(02)00524-1 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Jul 15;136(2):108-12. doi: 
      10.1016/s0165-4608(02)00524-1.