PMID- 12237239
OWN - NLM
STAT- MEDLINE
DCOM- 20020924
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 136
IP  - 2
DP  - 2002 Jul 15
TI  - Molecular cytogenetic characterization of a complex rearrangement involving 
      chromosomes 9 and 22 in a case of Ph-negative chronic myeloid leukemia.
PG  - 141-5
AB  - The "golden path", produced by the Human Genome Project effort, is composed of a 
      collection of overlapping and fully sequenced BAC/PAC clones covering almost 
      completely the human genome. These clones can be advantageously exploited as 
      fluorescence in situ hybridization (FISH) probes for the characterization of 
      rearrangements frequently found in tumors. Breakpoint characterization can be 
      further refined by generating additional smaller FISH probes through LONG-PCR 
      amplification of specific DNA segments, 5-10 kb in size, using appropriate 
      BAC/PAC probes as template. We report here an example of this approach that has 
      been used to characterize a complex Ph-negative chronic myeloid leukemia (CML 
      Ph-) case in which the BCR/ABL fusion gene was found located on chromosome 9.
FAU - Storlazzi, Clelia Tiziana
AU  - Storlazzi CT
AD  - DAPEG, Sezione di Genetica, Universita di Bari, Bari, Italy.
FAU - Anelli, Luisa
AU  - Anelli L
FAU - Surace, Cecilia
AU  - Surace C
FAU - Lonoce, Angelo
AU  - Lonoce A
FAU - Zagaria, Antonella
AU  - Zagaria A
FAU - Nanni, Mauro
AU  - Nanni M
FAU - Curzi, Paola
AU  - Curzi P
FAU - Rocchi, Mariano
AU  - Rocchi M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Chromosome Breakage
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*genetics
EDAT- 2002/09/19 10:00
MHDA- 2002/09/25 06:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2002/09/25 06:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
AID - S0165460802005307 [pii]
AID - 10.1016/s0165-4608(02)00530-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Jul 15;136(2):141-5. doi: 
      10.1016/s0165-4608(02)00530-7.