PMID- 12237875
OWN - NLM
STAT- MEDLINE
DCOM- 20030106
LR  - 20191210
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 198
IP  - 2
DP  - 2002 Oct
TI  - Detection of three common translocation breakpoints in non-Hodgkin's lymphomas by 
      fluorescence in situ hybridization on routine paraffin-embedded tissue sections.
PG  - 163-70
AB  - Non-random chromosomal translocations are specifically involved in the 
      pathogenesis of many non-Hodgkin's lymphomas and have clinical implications as 
      diagnostic and/or prognostic markers. Their detection is often impaired by 
      technical problems, including the distribution of the breakpoints over large 
      genomic areas. This study reports a fluorescence in situ hybridization (FISH) 
      method which allows the detection of specific chromosomal breakpoints in tissue 
      sections from routinely fixed, paraffin-embedded samples. Hybridization was 
      performed after demasking the DNA. Previously validated locus-specific probes 
      (cosmids, PACs) flanking the BCL1, BCL2 regions and similar new probes for the 
      MYC breakpoint region were used. The cases studied were five mantle cell 
      lymphomas (MCL) and five follicular lymphomas (FL), selected on the basis of a 
      previously proved t(11;14) and t(14;18) and five randomly chosen Burkitt's 
      lymphomas (BL), as well as 21 negative control samples. In all samples, 
      hybridization signals of sufficient intensity were obtained. Three different 
      algorithms were used to score the hybridization signals in tissue sections, two 
      of them taking into account the nuclei and their signal distribution indicative 
      of chromosomal break, and one only considering the colocalization or segregation 
      of the signals. In control tissues, these algorithms resulted in cut-off levels 
      of 9.1%, 1.3%, or 10.0%. In the 15 lymphoma samples the percentages of abnormal 
      cells/signals ranged from 28% to 80%, 13% to 49%, and 40% to 70%, respectively. 
      The results indicate that small locus-specific probes can be used in FISH for 
      regular detection of translocation breakpoints on routine paraffin tissue 
      sections.
CI  - Copyright 2002 John Wiley & Sons, Ltd.
FAU - Haralambieva, Eugenia
AU  - Haralambieva E
AD  - Department of Pathology, Leiden University Medical Centre, Leiden, The 
      Netherlands.
FAU - Kleiverda, Karin
AU  - Kleiverda K
FAU - Mason, David Y
AU  - Mason DY
FAU - Schuuring, Ed
AU  - Schuuring E
FAU - Kluin, Philip M
AU  - Kluin PM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
SB  - IM
MH  - Algorithms
MH  - Burkitt Lymphoma/genetics
MH  - Genes, bcl-1
MH  - Genes, bcl-2
MH  - Genes, myc
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Follicular/genetics
MH  - Lymphoma, Mantle-Cell/genetics
MH  - Lymphoma, Non-Hodgkin/*genetics
MH  - Paraffin Embedding
MH  - *Translocation, Genetic
EDAT- 2002/09/19 10:00
MHDA- 2003/01/08 04:00
CRDT- 2002/09/19 10:00
PHST- 2002/09/19 10:00 [pubmed]
PHST- 2003/01/08 04:00 [medline]
PHST- 2002/09/19 10:00 [entrez]
AID - 10.1002/path.1197 [doi]
PST - ppublish
SO  - J Pathol. 2002 Oct;198(2):163-70. doi: 10.1002/path.1197.