PMID- 12239249
OWN - NLM
STAT- MEDLINE
DCOM- 20030307
LR  - 20190607
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 13
IP  - 10
DP  - 2002 Oct
TI  - A Monocyte chemoattractant protein-1 (MCP-1) polymorphism and outcome after renal 
      transplantation.
PG  - 2585-9
AB  - Among the factors modulating transplant rejection and cardiovascular disease, 
      chemokines and their respective receptors deserve special attention. In this 
      respect, increased expression of MCP-1 and the corresponding receptor CCR2 have 
      been demonstrated in renal transplant rejection and coronary artery disease. The 
      impact of the MCP-1-2518G and CCR2-64I genotypes on renal allograft function was 
      investigated in 232 patients who underwent transplantation over an 11-yr period. 
      Genomic DNA was genotyped using PCR with sequence-specific primers followed by 
      restriction fragment length polymorphism analysis. Eighteen (7.8%) patients were 
      homozygous for the MCP-1-2518G mutation. The G/G allele of MCP-1 -2518 behaved as 
      a determinant for long-term allograft survival and resulted in reduction of the 
      mean graft survival, as compared with the heterozygous (A/G) or wild-type (A/A) 
      allele (67 +/- 14 versus 95 +/- 4 mo; Log rank P = 0.0052). The 64I mutation of 
      CCR2 had no effect on kidney graft failure (93 +/- 6 and 91 +/- 5 mo, 
      respectively; P = 0.81). None of the investigated polymorphisms showed a 
      significant shift in gene frequency in acute rejection and rejection-free groups. 
      In conjunction with these findings, peripheral blood mononuclear cells from 
      kidney transplant recipients carrying the G-allele were characterized by a 
      2.5-fold higher MCP-1 secretion (P < 0.05). In conclusion, recipients of renal 
      transplants homozygous for the -2518 G mutation of the MCP-1 gene are at risk for 
      premature kidney graft failure. This variant of MCP-1 may be a future predictor 
      for long-term kidney graft failure.
FAU - Kruger, Bernd
AU  - Kruger B
AD  - Klinik fur Innere Medizin II, University of Regensburg, Germany.
FAU - Schroppel, Bernd
AU  - Schroppel B
FAU - Ashkan, Rami
AU  - Ashkan R
FAU - Marder, Brad
AU  - Marder B
FAU - Zulke, Carl
AU  - Zulke C
FAU - Murphy, Barbara
AU  - Murphy B
FAU - Kramer, Bernhard K
AU  - Kramer BK
FAU - Fischereder, Michael
AU  - Fischereder M
LA  - eng
GR  - R01 AI 49289-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Chemokine CCL2/biosynthesis/*genetics
MH  - Female
MH  - Genotype
MH  - Graft Rejection/genetics
MH  - Graft Survival
MH  - Humans
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Monocytes/metabolism
MH  - *Polymorphism, Genetic
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2002/09/20 10:00
MHDA- 2003/03/08 04:00
CRDT- 2002/09/20 10:00
PHST- 2002/09/20 10:00 [pubmed]
PHST- 2003/03/08 04:00 [medline]
PHST- 2002/09/20 10:00 [entrez]
AID - 10.1097/01.asn.0000031701.53792.54 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2002 Oct;13(10):2585-9. doi: 
      10.1097/01.asn.0000031701.53792.54.