PMID- 12239403
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 8
IP  - 5
DP  - 1996 May
TI  - Localization of T-DNA Insertions in Petunia by Fluorescence in Situ 
      Hybridization: Physical Evidence for Suppression of Recombination.
PG  - 823-830
AB  - Using fluorescence in situ hybridization (FISH) with metaphase preparations, we 
      localized a 4-kb single-copy T-DNA sequence in a group of petunia transformants. 
      The selected T-DNAs previously had been shown to be linked to the phenotypic 
      marker FI on chromosome II. Linkage analysis had revealed that recombination 
      around the FI locus is suppressed in a wide cross relative to an inbred 
      recombination assay. The localization of six FI-linked T-DNAs and the FI locus 
      itself, using FISH, revealed a number of aspects of recombination in petunia: (1) 
      the central region of chromosome II showed at least a 10-fold suppression of 
      recombination in wide crosses relative to the distal region; (2) recombination in 
      wide hybrids over two-thirds of the chromosome was extremely low; and (3) 
      recombination between completely homologous chromosomes in an inbred cross also 
      was suppressed in the central region. In addition, the T-DNAs were not evenly 
      distributed along the chromosome, suggesting a possible preference for a distal 
      position for T-DNA integration. Implications for such a preference are discussed.
FAU - Ten Hoopen, R
AU  - Ten Hoopen R
AD  - Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of 
      Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands.
FAU - Robbins, T P
AU  - Robbins TP
FAU - Fransz, P F
AU  - Fransz PF
FAU - Montijn, B M
AU  - Montijn BM
FAU - Oud, O
AU  - Oud O
FAU - Gerats, AGM
AU  - Gerats A
FAU - Nanninga, N
AU  - Nanninga N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
PMC - PMC161141
EDAT- 1996/05/01 00:00
MHDA- 2002/09/20 10:00
PMCR- 1997/05/01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 2002/09/20 10:00 [medline]
PHST- 1996/05/01 00:00 [entrez]
PHST- 1997/05/01 00:00 [pmc-release]
AID - 8/5/823 [pii]
AID - 10.1105/tpc.8.5.823 [doi]
PST - ppublish
SO  - Plant Cell. 1996 May;8(5):823-830. doi: 10.1105/tpc.8.5.823.