PMID- 12241011
OWN - NLM
STAT- MEDLINE
DCOM- 20030701
LR  - 20161018
IS  - 1434-6621 (Print)
IS  - 1434-6621 (Linking)
VI  - 40
IP  - 7
DP  - 2002 Jul
TI  - Isolation of fetal nucleated red blood cells from maternal blood in normal and 
      aneuploid pregnancies.
PG  - 667-72
AB  - Fetal nucleated red blood cells (NRBC) have been widely reported in maternal 
      blood during pregnancy. However, there is no consensus with regard to their 
      presence in all pregnancies. Therefore, the usefulness of developing a fetal 
      NRBC-based noninvasive method suitable for clinical prenatal diagnosis remains 
      uncertain. Fluorescence in situ hybridization (FISH) method was used to evaluate 
      the ability of one of our own monoclonal antibodies (mAb), 2B7.4, to isolate 
      fetal NRBC from maternal blood by magnetic activated cell sorting (MACS). Our mAb 
      was able to isolate from 25 to 822 NRBC from all of the 45 maternal blood samples 
      included in this study. A correct diagnosis was achieved in 21 out of 24 
      pregnancies carrying trisomic fetuses (87.5%), with a fetal/maternal NRBC 
      frequency of 8.4%. In contrast, a significantly lower percentage of fetal NRBC 
      (0.2%) was observed in 22% of pregnancies carrying a chromosomally normal male 
      fetus, that were correctly predicted. In conclusion, using 2B7.4 mAb we succeeded 
      in isolating NRBC from the maternal blood samples, but most of the isolated cells 
      were maternal in origin. Nevertheless, a higher number of fetal NRBC was found in 
      the peripheral blood of pregnant women carrying aneuploid fetuses, which could 
      allow development of a screening method for prenatal diagnosis of fetal 
      aneuploidies.
FAU - Prieto, Belen
AU  - Prieto B
AD  - Servicio de Analisis Clinicos, Hospital San Agustin, Aviles, Asturias, Spain.
FAU - Candenas, Mercedes
AU  - Candenas M
FAU - Venta, Rafael
AU  - Venta R
FAU - Ladenson, Jack H
AU  - Ladenson JH
FAU - Alvarez, Francisco V
AU  - Alvarez FV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
SB  - IM
MH  - Aneuploidy
MH  - *Erythroblasts
MH  - Female
MH  - Fetomaternal Transfusion/*blood
MH  - Humans
MH  - Immunomagnetic Separation
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Trisomy/*diagnosis
EDAT- 2002/09/21 10:00
MHDA- 2003/07/02 05:00
CRDT- 2002/09/21 10:00
PHST- 2002/09/21 10:00 [pubmed]
PHST- 2003/07/02 05:00 [medline]
PHST- 2002/09/21 10:00 [entrez]
AID - 10.1515/CCLM.2002.114 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2002 Jul;40(7):667-72. doi: 10.1515/CCLM.2002.114.