PMID- 12269763
OWN - NLM
STAT- MEDLINE
DCOM- 20030417
LR  - 20191106
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 26
IP  - 3
DP  - 2002
TI  - Targeting multiple genetic aberrations in isolated tumor cells by spectral 
      fluorescence in situ hybridization.
PG  - 171-9
AB  - PURPOSE: Tumorigenesis is characterized by the stepwise accumulation of multiple 
      genetic changes that modify specific growth controls and cell survival. 
      Conventional fluorescence in situ hybridization (FISH) assays reliably target one 
      to three probes in a single hybridization. Simultaneous detection of more than 
      three chromosomal or gene targets should increase the overall power of molecular 
      cytogenetics by permitting detection of multiple genetic aberrations at the 
      single cell level. METHOD: Spectral FISH (S-FISH) is an innovative molecular 
      cytogenetic approach that can target many specific chromosomal aberrations in 
      interphase and metaphase cells in a single hybridization, using combinatorial 
      fluorescence and digital imaging microscopy. RESULTS: S-FISH is a reliable means 
      to identify disease-specific aberrations at the DNA level in individual tumor 
      cells in hematopoietic disorders and malignant melanoma. CONCLUSION: S-FISH is a 
      sensitive assay for the diagnosis and monitoring of disease-specific or 
      patient-specific genetic aberrations, with significant clinical application in 
      oncology for early detection of new or re-emerging abnormal clones, allowing for 
      earlier therapeutic intervention.
FAU - Slovak, Marilyn L
AU  - Slovak ML
AD  - Department of Cytogenetics, City of Hope National Medical Center, Duarte, CA 
      91010, USA. mslovak@coh.org
FAU - Zhang, Feiyu
AU  - Zhang F
FAU - Tcheurekdjian, Lucene
AU  - Tcheurekdjian L
FAU - Bobadilla, Dolores
AU  - Bobadilla D
FAU - Bedell, Victoria
AU  - Bedell V
FAU - Arber, Daniel A
AU  - Arber DA
FAU - Persons, Diane L
AU  - Persons DL
FAU - Sosman, Jeffrey A
AU  - Sosman JA
FAU - Murata-Collins, Joyce L
AU  - Murata-Collins JL
LA  - eng
GR  - CA30206/CA/NCI NIH HHS/United States
GR  - CA32102/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
SB  - IM
MH  - Acute Disease
MH  - *Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myeloid/*genetics
MH  - Male
MH  - Melanoma/*genetics/secondary
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*genetics/secondary
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2002/09/25 06:00
MHDA- 2003/04/18 05:00
CRDT- 2002/09/25 06:00
PHST- 2002/09/25 06:00 [pubmed]
PHST- 2003/04/18 05:00 [medline]
PHST- 2002/09/25 06:00 [entrez]
AID - S0361-090X(02)00063-6 [pii]
AID - 10.1016/s0361-090x(02)00063-6 [doi]
PST - ppublish
SO  - Cancer Detect Prev. 2002;26(3):171-9. doi: 10.1016/s0361-090x(02)00063-6.