PMID- 12270111
OWN - NLM
STAT- MEDLINE
DCOM- 20021107
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 297
IP  - 3
DP  - 2002 Sep 27
TI  - Human osteoclasts express oxytocin receptor.
PG  - 442-5
AB  - Increasing evidences demonstrated many new targets for the hypothalamic hormone 
      oxytocin, as the regulation of food balance and in some cases of leptin 
      secretion. Considering that leptin is a potent inhibitor of bone formation and 
      that oxytocin receptors (OTR) were detected in normal human osteoblasts, we 
      investigated if OTR was expressed by human osteoclasts (hOCs) and the effect of 
      the hormone on these cells. Here, we demonstrate by immunofluorescence and by 
      Western blot analysis the expression of OTR by fully differentiated hOCs and by 
      their precursors (pOCs). We also show that the receptor is functional, as OT 
      treatment induces an increase of [Ca(2+)](i), and that the hormone may affect 
      osteoclastogenesis, since it increases the number of pre-osteoclasts.
FAU - Colucci, Silvia
AU  - Colucci S
AD  - Department of Human Anatomy and Histology, University of Bari, Policlinico-P.zza 
      G. Cesare 11, Bari 70 124, Italy. s.colucci@anatomia.uniba.it
FAU - Colaianni, Graziana
AU  - Colaianni G
FAU - Mori, Giorgio
AU  - Mori G
FAU - Grano, Maria
AU  - Grano M
FAU - Zallone, Alberta
AU  - Zallone A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Receptors, Oxytocin)
RN  - 50-56-6 (Oxytocin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/metabolism
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Cytosol/drug effects/metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Kinetics
MH  - Osteoclasts/cytology/drug effects/*metabolism
MH  - Oxytocin/pharmacology
MH  - Receptors, Oxytocin/*metabolism
EDAT- 2002/09/25 06:00
MHDA- 2002/11/26 04:00
CRDT- 2002/09/25 06:00
PHST- 2002/09/25 06:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/09/25 06:00 [entrez]
AID - S0006291X02020090 [pii]
AID - 10.1016/s0006-291x(02)02009-0 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2002 Sep 27;297(3):442-5. doi: 
      10.1016/s0006-291x(02)02009-0.